Sequences from confirmation amplicon


Confirmation sequences for line 261E09 and hit At3g46400 are shown.
Line ID 261E09
Locus At3g46400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17075603
Primers used for PCR PP23 - 8474, show primer details
   
Sequence Name 14-K102723-0022-261-E09C-8409
GenBank Accession LN492460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102723-0022-261-E09C-8409
TATGTAGAAATATTGTTGTCATATGAGTTGACCACTTTTTCTTTGTGAAAACGAAAATGA
GAATCAGTACCAATATGGCGACAACTATCGCAGAAGCAGCTAACGCAGCAATCATCATTG
GAAATTTATTTTTTGGGACACATGATAAACACGTATTATCACCATCAACACTACAAAACA
AAATAATTCATCTACCTTTTAGTATTTTGAATGCAAAAGCCTTGTGCCACAAGGGAGATC
TTGAACAAAAAAAATGTGTGCGAATGGATTTCCTTCTTACAATATTTGTAGTCCTTTCTT
TTCTCTATCACGAAGAGTATTTGGAATTGAACCATTCAGTTTGGTCTTCCT
   
Sequence Name 30-K102728-0022-261-E09C-PP23
GenBank Accession LN499668 [GenBank]
Sequencing primer gene specific primer, PP23: 5'-GAGGAAGAACAAACTGAATGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K102728-0022-261-E09C-PP23
AGAAAGAAGGACTACAATATTGTAAGAAGGAAATCCATTCGCACACATTTTTTTTGTTCA
AGATCTCCCTTGTGGCACAAGGCTTTTGCATTCAAAATACTAAAAGGTAGATGAATTATT
TTGTTTTGTAGTGTTGATGGTGATAATACGTGTTTATCATGTGTCCCAAAAAATAAATTT
CCAATGATGATTGCTGCGTTAGCTGCTTCTGCGATAGTTGTCGCCATATTGGTACTGATT
CTCATTTTCGTTTTCACAAAGAAAAAGTGGTCAACTCATATGACAACAATATTTCTACAT
ATTAATTACCTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTCA
Primers used for PCR 14mg - 3144, show primer details
   
Sequence Name 61-K118471-0022-261-E09C-14mg
GenBank Accession LN510624 [GenBank]
Sequencing primer gene specific primer, 14mg: 5'-AAGAAGTTCGACCTACAAACCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K118471-0022-261-E09C-14mg
TGAATATTAAACAAAAATACATTTGTGTGATTTTTTTTCTTTTCATATTGTAGGAAGATC
TATGGCATATCATACCTCTGCCTTGAAGTGTTTATAGCCTTGCGATGATGATTGTGATAG
TACTTTAACCGCTACTTGCTCTACATTTTTCAGATAACCATGGTAGACTATGCCAAACCC
TCCTTCACCTAGAGCTTTTTCGAATTTTTTTGTCATTTCTACAACTTCTGAATAAGCAAA
TCTTCTCCTTTTTGTCTTGATTAATTGTTCGGATATAGTCTTGGACATAATATCCATTGT
TGGCAAAATTACTGATGGGGATAGATATATGCAAATAACAATCTAGTGAGACAACAATAT
CCATGACACCTTATAATATATAAATTGTTGCGTTAATAATTAGGTAATTAATATTTCAAA
CACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATA
   
Sequence Name 29-K118470-0022-261-E09C-35St
GenBank Accession LN499486 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118470-0022-261-E09C-35St
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAA
TATTAATTACCTAATTATTAACGCAACAATTTATATATTATAAGGTGTCATGGATATTGT
TGTCTCACTAGATTGTTATTTGCATATATCTATCCCCATCAGTAATTTTGCCAACAATGG
ATATTATGTCCAAGACTATATCCGAACAATTAATCAAGACAAAAAGGAGAAGATTTGCTT
ATTCAGAAGTTGTAGAAATGACAAAAAAATTCGAAAAAGCTCTAGGTGAAGGAGGGTTTG
GCATAGTCTACCATGGTTATCTGAAAAATGTAGAGCAAGTAGCGGTTAAAGTACTATCAC
AATCATCATCGCAAGGCTATAAACACTTCAAGGCAGAGGTATGATATGCCATAGATCTTC
CTACAATATGAAAAGAAAAAAAATCACACAAATGTATTTTTGTTTTATATATTTCACATC
ATGTCAAACAAATGTGTTTTGTTATTGTTGGTTTGTAGGCGAAAACTTCTT
Primers used for PCR 13mg - 8474, show primer details
   
Sequence Name 60-K118471-0022-261-E09C-13mg
GenBank Accession LN510373 [GenBank]
Sequencing primer gene specific primer, 13mg: 5'-TTCAATTCCAAATACTCTTCGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118471-0022-261-E09C-13mg
ATTTCGCACACATTTTTTTTGTTCAAGATCTCCCTTGTGGCACAAGGCTTTTGCATTCAA
AATACTAAAAGGTAGATGAATTATTTTGTTTTGTAGTGTTGATGGTGATAATACGTGTTT
ATCATGTGTCCCAAAAAATAAATTTCCAATGATGATTGCTGCGTTAGCTGCTTCTGCGAT
AGTTGTCGCCATATTGGTACTGATTCTCATTTTCGTTTTCACAAAGAAAAAGTGGTCAAC
TCATATGACAACAATATTTCTACATATTAATTACCTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 28-K118470-0022-261-E09C-8409
GenBank Accession LN499050 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118470-0022-261-E09C-8409
TTTAGGTAATTAATATGTAGAAATATTGTTGTCATATGAGTTGACCACTTTTTCTTTGTG
AAAACGAAAATGAGAATCAGTACCAATATGGCGACAACTATCGCAGAAGCAGCTAACGCA
GCAATCATCATTGGAAATTTATTTTTTGGGACACATGATAAACACGTATTATCACCATCA
ACACTACAAAACAAAATAATTCATCTACCTTTTAGTATTTTGAATGCAAAAGCCTTGTGC
CACAAGGGAGATCTTGAACAAAAAAAATGTGTGCGAATGGATTTCCTTCTTACAATATTT
GTAGTCCTTTCTTTTCTCTATCACGAAGAGTATTGGGAAATTGA

Last Updated on 06.12.2018 16:11