Sequences from confirmation amplicon


Confirmation sequences for line 261G08 and hit At1g05540 are shown.
Line ID 261G08
Locus At1g05540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1639938
Primers used for PCR 17rn - 8474, show primer details
   
Sequence Name 73-K115963-0022-261-G08C-17rn
GenBank Accession LN512962 [GenBank]
Sequencing primer gene specific primer, 17rn: 5'-TTGTTCCCTCTTCTGTTTCTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115963-0022-261-G08C-17rn
GATGTGAGAGTACCTGCACACGCATATAAGACCCAAATTAGCATTGACCAAGATTTCTGT
CTACATGTTAGACTCGCACGTTACCATTTGACTAAGAAACTTTGGCCGGAAGGTGACTCC
ACCCAATGGTCCTCCTTGAAACAAGAATTCAACAGCTCCCACTCGGACTGTGGCAACTCA
GGGAGATTAAGAAACACCAACTCATTGAACTTAGGATCGTTATCGAAGTGGAGATCCCAA
GAGCACAAGTAATTGCCTCCAGGAGCAGGCAAATAAAACCTTTGATCTCTCTTCGAGTAC
ATAAGATTTGAGTTTTCCGAGCAGATGAAAGGGATTTGTTTGTTAGTCCAACCGTGGTCA
CGACCGGGCCTGCACACACTCAGTTGGCTACCCAAGAAATTGATAGCCACGACACAGTCT
TCTTCTTCATTGTCTTGGTGAGGAGAAGATGACGACATTGCAAATGAGTATATATTCAAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCG
   
Sequence Name 73-K115962-0022-261-G08C-8409
GenBank Accession LN512961 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115962-0022-261-G08C-8409
CATTTCATTGAATATTGAATATATACTCATTTGCAATGTCGTCATCTTCTCCTCACCAAG
ACAATGAAGAAGAAGACTGTGTCGTGGCTATCAATTTCTTGGGTAGCCAACTGAGTGTGT
GCAGGCCCGGTCGTGACCACGGTTGGACTAACAAACAAATCCCTTTCATCTGCTCGGAAA
ACTCAAATCTTATGTACTCGAAGAGAGATCAAAGGTTTTATTTGCCTGCTCCTGGAGGCA
ATTACTTGTGCTCTTGGGATCTCCACTTCGATAACGATCCTAAGTTCAATGAGTTGGTGT
TTCTTAATCTCCCTGAGTTGCCACAGTCCGAGTGGGAGCTGTTGAATTCTTGTTTCAAGG
AGGACCATTGGGTGGAGTCACCTTCCGGCCAAAGTTTCTTAGTCAAATGGTAACGTGCGA
GTCTAACATGTAGACAGAAATCTTGGTCAATGCTAATTTGGGTCTTATATGCGTGTGCAG
GTACTCTCACATCCCTTCTCAACGATACAAAGATCCGATATTGATGGTGCTTAGAGAGGA
TGAAGAAACA

Last Updated on 06.12.2018 16:11