Sequences from confirmation amplicon


Confirmation sequences for line 263D04 and hit At3g20820 are shown.
Line ID 263D04
Locus At3g20820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7281793
Primers used for PCR KS58 - 8474, show primer details
   
Sequence Name 35-K101501-0022-263-D04C-8409
GenBank Accession LN501767 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101501-0022-263-D04C-8409
GGCATCTTCACGATGGCCTTTCAGGTATCTTCCCTTGTAGCAAGTTCCGGCTCAAATTCA
GATTCATCACCGACGACGTCATCAGAGTTTGAGGTATCTCGCCGGAGATTTTGTTTCCGT
CGAGGTTAAGTGTGGCTAGTACCGACATACGTCCTAGAGACGGTGGGATTGTACCGTAGA
GTTGGTTACCGGAGAGATCCACGTCAGCTAACCGGTAGATATTGGTTAATGATTCTGGGA
TCCGACCGGTTATTCGGTTACCGGATAATAACGCACGGCTGAGCATTTTTAATCTTCCGA
CGTCCGACGGGATTACACCGGAGATTAAGTTGTTACGGAGATCTAAATGCATTAAGCTAG
AGAGATTGGTTAATGATTTTGGAATTGAACCGGAGATTCGGTTATCGGCTACGTTTAAAA
CAGC
   
Sequence Name 35-K101505-0022-263-D04C-KS58
GenBank Accession LN501768 [GenBank]
Sequencing primer gene specific primer, KS58: 5'-AGCTGTTTTAAACGTAGCCGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101505-0022-263-D04C-KS58
CAATCATTACCAATCTCTCTAGCTTAATGCATTTAGATCTCCGTAACAACTTAATCTCCG
GTGTAATCCCGTCGGACGTCGGAAGATTAAAAATGCTCAGCCGTGCGTTATTATCCGGTA
ACCGAATAACCGGTCGGATCCCAGAATCATTAACCAATATCTACCGGTTAGCTGACGTGG
ATCTCTCCGGTAACCAACTCTACGGTACAATCCCACCGTCTCTAGGACGTATGTCGGTAC
TAGCCACACTTAACCTCGACGGANACAAAATCTCCGGCGAGATACCTCAAACTCTGATGA
CGTCGTCGGTGATGAATCTGAATTTGAGCCGGAACTTGCTACAAGGGAAGATACCTGAAA
GGCCATCGTTGAAGATGCCTCTACATGTCCGGATAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAG

Last Updated on 06.12.2018 16:11