Sequences from confirmation amplicon


Confirmation sequences for line 264F05 and hit At1g50140 are shown.
Line ID 264F05
Locus At1g50140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18578580
Primers used for PCR 17eq - 8474, show primer details
   
Sequence Name 51-K111119-0022-264-F05C-17eq
GenBank Accession LN507537 [GenBank]
Sequencing primer gene specific primer, 17eq: 5'-GCAAATCAGAACATATCAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111119-0022-264-F05C-17eq
CGAAGGGAACGTCTTTGGTTATTACTGGACGTAGTAAGGGAAAGACTCCCAAGAGAGCTG
TATAGTTTCCCAAGGTTGAATTCTCCGCAAGAACTCATTCTTCAATCTTTCTCGTTTATT
AAGAAAAGGTGACTCCTTTCTGCTTTCACTTGCACGCTTCTCATTGTTGAGCCATTCTAT
TTGGTCTTGTACTCCAAGTTGAGCGTGAGAATCAAAATGCTCAACACCGACCGTGCTCTT
CTCTTTTCCCATCTTGTCTTTATTCAGAGGGAAACGGCTGTCCTCACTAGCATTATTTCC
ATCACCTTCAGAACTAAACCGACGCANTTGGCTATTCCAAGAATAACTTAAATAATCTCT
AGAGGCAATAGCACCATGAGAATGATGGTTTCTTCTCAAATGATCTGTTAGTATACTTGT
GGTGGATCTATAAGATTGGGAACCGGTGTGGTCATGAAGCCATTGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 35-K111118-0022-264-F05C-8409
GenBank Accession LN501872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111118-0022-264-F05C-8409
CATTTACATTGAATTATCCTGCAATGGCTTCATGACCAGCACCGGTTCCCAATCTTATAG
ATCCACCACAAGTATACTAACAGATCATTTGAGAAGAAACCATCATTCTCATGGTGCTAT
TGCCTCTAGAGATTATTTAAGTTATTCTTGGAATAGCCAATTGCGTCGGTTTAGTTCTGA
AGGTGATGGAAATAATGCTAGTGAGGACAGCCGTTTCCCTCTGAATAAAGACAAGATGGG
AAAAGAGAAGAGCACGGTCGGTGTTGAGCATTTTGATTCTCACGCTCAACTTGGAGTACA
AGACCAAATAGAATGGCTCAACAATGAGAAGCGTGCAAGTGAAAGCAGAAAGGAGTCACC
TTTTCTTAATAAACGAGAAAGATTGAAGAATGAGTTCTTGCGGAGAATTCAACCTTGGGA
AACTATACAGCTCTCTTGGGAGTCTTTCCCTTACTACGTCCAGTAATAACCAAAGACTGT
TCCCTTCTGTTATCGTGTTACTTTCTTCTAGTCATTTCCATTTTTTGCTGATATGTTTGG
GATTTG

Last Updated on 06.12.2018 16:11