Sequences from confirmation amplicon


Confirmation sequences for line 266A04 and hit At3g62680 are shown.
Line ID 266A04
Locus At3g62680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23183755
Primers used for PCR 14de - 8474, show primer details
   
Sequence Name 49-K109414-0022-266-A04C-14de
GenBank Accession LN506869 [GenBank]
Sequencing primer gene specific primer, 14de: 5'-TGTTTAGTAGACTCGTGTTGACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K109414-0022-266-A04C-14de
TAATATTTTTTTATCTTCNACAAATAATACTCGTTAAATTAAATTTCTCATATAAAAGTA
GTCGCATAAACAAAAAAAAAAAAAAAAATTTTCCCCTGGCATTTTTTTTATTGGAAAGGA
AAACCTTACCCAAAGGGATAATTGGCTTGGAAACCAAAGGAAACCGGACCAAACAACCAA
AAAAACTTTTTCAGGGGAAAAATTCCCAAAAAAAAATTGGTTAAAGGGGCCCCCTTATTT
AAGGCGGGCAAAACCATAACCTCCCCCCCCAAAATAGGACCCCCCCAATCATTTTTTGGG
AGGGGGGGGGCCCCCCTTGGACCCAGTGTATAAAAAGGGCCCAACCCTAAAAACCCAAAC
TTGGGCCTTTTTTAGGATTTCGGGGGGGGGCCCCCTGGGACCCAGGGCCGGGATATTTCC
ATTTGAAAAGGGCTCCTTGCCCGGAAAACCTCCTGGGACCCCCATGGAGTTTAAGGGCCA
ATTGGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTCCATTTCAAAAAGGTAAATTCCC
CCCCCGTTTTAAAAAAA
   
Sequence Name 33-K109413-0022-266-A04C-8409
GenBank Accession LN501032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K109413-0022-266-A04C-8409
CATTTACAATTGAATATATCCTGACACTGGTCCCAAGGCTGCCCCCGCCAGAACTCCTAA
CAACGACCAATGTTAGGTTCTTTAGCGTCGGACCTTTCTACTACACTGGTCCCAAGGCTG
CCCCCGCCACTCCCAAATACTGATCGTGATGATCATATTATGATGATGATGATTATGATT
TTGCATGCATTATATAAGAGGTCACTTTAATCAATTTATTATTTGGAATTTTTTCCCTTG
AAAATGTTCTTTTGTTTGTTTGTTCCGATTTCATTTGATTCCAATGCAATCTATCCTTTT
GGTTATGCTTTTCATTTCATATAATAAAATTGCAAGAGTAAATTCTTTTTTTTTTTTTTT
GTTTAGGCCACCCCTTTTTTTTGAAAATTTTATTTTAACGAGTTTTTTTTGTTTAAAGGA
AAAAAAAAATATTTTTATTTTTTGGCCTTGGGGCCAACCCAATTCAACAAAAAAA

Last Updated on 06.12.2018 16:11