Sequences from confirmation amplicon


Confirmation sequences for line 266H03 and hit At1g20570 are shown.
Line ID 266H03
Locus At1g20570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7123223
Primers used for PCR 14vh - 8474, show primer details
   
Sequence Name 18-K118126-0022-266-H03C-14vh
GenBank Accession LN494501 [GenBank]
Sequencing primer gene specific primer, 14vh: 5'-CAAAATTTGTCGGAACAAACTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118126-0022-266-H03C-14vh
TTTCTTCTCAGATGACACCAAAATTCTAGAAACGTTATTTCCTTTTCCTACATTGCTGCC
GTCATTCCAGGTACTTAGATACTGATCATTTTATGTCCATTTCTTCACTTTTATCCCATG
TGCAATAGTTTATGATAGCGGAGTTTGAAATCTTTGTGATCAACAAAAAAGTCGAAGCTC
CATATGTCAGAATTCCTACCTTACCAAAAGAATAGTACACTTCCTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K118125-0022-266-H03C-8409
GenBank Accession LN487227 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118125-0022-266-H03C-8409
CATTTCATTGAAGGAAGTGTACTATTCTTTTGGTAAGGTAGGAATTCTGACATATGGAGC
TTCGACTTTTTTGTTGATCACAAAGATTTCAAACTCCGCTATCATAAACTATTGCACATG
GGATAAAAGTGAAGAAATGGACATAAAATGATCAGTATCTAAGTACCTGGAATGACGGCA
GCAATGTAGGAAAAGGAAATAACGTTTCTAGAATTTTGGTGTCATCTTGAGAAGAAAGAT
ATTCAGATCTACCGAATTGGAATCCATATTTGTAGTTTGTTCCGACAAATTTTG
Primers used for PCR 87wb - 3144, show primer details
   
Sequence Name 26-K118238-0022-266-H03C-87wb
GenBank Accession LN498125 [GenBank]
Sequencing primer gene specific primer, 87wb: 5'-GGAAACTAATACGGTTGTCGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118238-0022-266-H03C-87wb
CCCTATCATTACNTATTGATGAAAGCATCCACAAAATTGAGGAGCTTTTGTTCCAGTAAA
CAGTGGTGCTTGCGGATTTTTGTTGCAGAGCCTTTACCCTATAATACATAAACACCAAAG
TCATTAAGTTTATCAAACTAAACCACATCATTATGACTCCTCAGTACATTACGTATATCA
AAAGACTACTCAGGCTATCTGAGATTGGATTCGGATGTCAAATGAAGCAGTCTAAATGGA
AAGAAATGGTTTGAGAGATATCAAAGACCTTCCACATCAACCTTCGTGCCTTATCAAGCA
CATATTTTGCTCGCTTGACCTTCAACAAAAATCCCATCACCTGTTTTCATTTAAACAATT
AACACATAGAACATCACACTGTTTCCCCAAAAAAATAGCTGGATGAGAAAGGGTTACCTG
GTTATACTTCTTAATGGCCTCGCTGTTCGCAATAAGCTCAAGCGGCCACGGCACCTTAAA
AGGAAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC
TGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 12-K118237-0022-266-H03C-35St
GenBank Accession LN491787 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118237-0022-266-H03C-35St
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTCCTTTTAAGGT
GCCGTGGCCGCTTGAGCTTATTGCGAACAGCGAGGCCATTAAGAAGTATAACCAGGTAAC
CCTTTCTCATCCAGCTATTTTTTTGGGGAAACAGTGTGATGTTCTATGTGTTAATTGTTT
AAATGAAAACAGGTGATGGGATTTTTGTTGAAGGTCAAGCGAGCAAAATATGTGCTTGAT
AAGGCACGAAGGTTGATGTGGAAGGTCTTTGATATCTCTCAAACCATTTCTTTCCATTTA
GACTGCTTCATTTGACATCCGAATCCAATCTCAGATAGCCTGAGTAGTCTTTTGATATAC
GTAATGTACTGAGGAGTCATAATGATGTGGTTTAGTTTGATAAACTTAATGACTTTGGTG
TTTATGTATTATAGGGTAAAGGCTCTGCAACAAAAATCCGCAAGCACCACTGTTTACTGG
AACAAAAGCTCCTCAATTTTGTGGATGCTTTTCATCAATACGTAATGGATAGGGTAATGT
CTAATACTGGATAATTGGTTATAATTACCGACAACCGTATAAGTTT

Last Updated on 06.12.2018 16:11