Genotyping and primer details


Genotyping and primer details for 266H03 - At1g20570
Line ID 266H03
Locus At1g20570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7123223 of line 266H03 Chr1:7123223
   
Insertion allele amplicon 8474 - 14vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14vh: 5'-CAAAATTTGTCGGAACAAACTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7124410
   
Insertion allele amplicon 3144 - 87wb
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 87wb: 5'-GGAAACTAATACGGTTGTCGGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7122681
   
wt amplicon (over InsPos) 47wd - 14vh
wt amplicon start Chr1:7123321
wt amplicon end Chr1:7124410
wt amplicon size 1090 bp
Locus specific primer 14vh: 5'-CAAAATTTGTCGGAACAAACTACA-3'
2nd locus specific primer 47wd: 5'-GAATTTGAGAGATTCAAGGCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11