Sequences from confirmation amplicon


Confirmation sequences for line 268C08 and hit At5g38250 are shown.
Line ID 268C08
Locus At5g38250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15281763
Primers used for PCR 54cp - 8474, show primer details
   
Sequence Name 24-K108475-0022-268-C08C-54cp
GenBank Accession LN497025 [GenBank]
Sequencing primer gene specific primer, 54cp: 5'-TCTTCACAGTTTCCCTTTGAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108475-0022-268-C08C-54cp
CACTTTCACATCGCGTATCTCCCTCTATATACAGTTCCAATCCTCCTCGTCCACTACCTT
TGTAAATGATTTTGTAATTTTCTTGATTTGTTTATAATTGTACCCTCTCAGGGTTCCAAG
CGCCTCGAGGTGCTGAATTCTCGCAGCCTTTGCTATTCTTCTCTCGTTAAGATAAAATGC
TAGAAACAGCACCACACATACACATAGTATTGACCCTAGAACTAAAAAAATTGAAAACAA
CCTTAGTTTCAGTCCCGCAAAAGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 08-K108474-0022-268-C08C-8409
GenBank Accession LN489767 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K108474-0022-268-C08C-8409
CATTGAACTTTTGCGGGACTGAACTAAGGTTGTTTTCAATTTTTTTAGTTCTAGGGTCAA
TACTATGTGTATGTGTGGTGCTGTTTCTAGCATTTTATCTTAACGAGAGAAGAATAGCAA
AGGCTGCGAGAATTCAGCACCTCGAGGCGCTTGGAACCCTGAGAGGGTACAATTATAAAC
AAATCAAGAAAATTACAAAATCATTTACAGAAGTAGTTGGACGAGGAGGATTTGGAACTG
TATATAGAGGGAGATTACGCGATGGTCGAAAGGTTGCCGTGAAGGTCTTGAAAGATTCAA
AGGGAAACTGTGAAGA

Last Updated on 06.12.2018 16:11