Sequences from confirmation amplicon


Confirmation sequences for line 268H04 and hit At5g54710 are shown.
Line ID 268H04
Locus At5g54710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22229144
Primers used for PCR 18sj - 8474, show primer details
   
Sequence Name 61-K116453-0022-268-H04C-18sj
GenBank Accession LN510603 [GenBank]
Sequencing primer gene specific primer, 18sj: 5'-TGGCAAGGACACTATTAGATGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K116453-0022-268-H04C-18sj
TGGATAAGATGGTTTGACCCCTTTGCATCGCGCGGTGATAAACGGTTCAGTTGAAATCCT
AAAGGAGTTTCTATGCAAGGCTCCTTCGTCTTTCAACATAACCACACAAGGAACAATCGA
AACAGTTTTCCACTTAGCTGCGAAATACCAAAAAACCAAAGCATTCATCTTCATGGCCCA
GAGTGCAAACATACGCCAACTTCTTTACAGCCTCGACGCAGAGGACAACACTGTACTTCA
TGTTGCAACAATATGGAGACTCCACTTCTGGACAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGATTATT
   
Sequence Name 28-K116452-0022-268-H04C-8409
GenBank Accession LN499001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116452-0022-268-H04C-8409
ACATTGAATATATCCTGTCCAGAAGTGGAGTCTCCATATTGTTGCAACATGAAGTACAGT
GTTGTCCTCTGCGTCGAGGCTGTAAAGAAGTTGGCGTATGTTTGCACTCTGGGCCATGAA
GATGAATGCTTTGGTTTTTTGGTATTTCGCAGCTAAGTGGAAAACTGTTTCGATTGTTCC
TTGTGTGGTTATGTTGAAAGACGAAGGAGCCTTGCATAGAAACTCCTTTAGGATTTCAAC
TGAACCGTTTATCACCGCGCGATGCAAAGGGGTCAAACCATCTTTATCCACCTTTTCTGC
AATTTCTTGATTAACATCAACATCTAATATGTTCCTTGCCAA

Last Updated on 06.12.2018 16:11