Sequences from confirmation amplicon


Confirmation sequences for line 269F02 and hit At2g35340 are shown.
Line ID 269F02
Locus At2g35340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14875574
Primers used for PCR 77cs - 8474, show primer details
   
Sequence Name 73-K110680-0022-269-F02C-77cs
GenBank Accession LN512920 [GenBank]
Sequencing primer gene specific primer, 77cs: 5'-TTGGTCGATCTGATATCTTGACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K110680-0022-269-F02C-77cs
GAAAGTACCAAGAAGAAGAAATGTAGGAGAGGTAATTGCATCTTACTGTACCCTTCTTAA
CTTTTTGGCTATTTTTTAGAATGCTTCAAGTGTTCTTGATGATTTTAGTTTTCCTTCTTC
TGAATTATTTGTACTTATCTCTTTTTTTTCAACCTCGTTGGAGTACAGTATAGAATGCCG
GATGCATATGATCAACAGGGAAGTGTTGATCAGGAGAAGAGGTTTGCTGTTTCTGTACAA
CGGTACAGGTGAGATGAGAAAGAATGCATGACTTGGAATCCATAACATTCTACTTTGCTT
ACACTTTTAAAGATCACTCTTCTTTGTGTTGTAAAAGGCTAACGTATGTTCTAAATTATC
TTATTTGATTAGGGACATGGGTTCCGCAGAGAAAATGAACCCCTTTGCAGAGCAAGAAGC
TTGGGAGGACCATCAGATTGGTAGTATTCTGTTTAGCGCTTTTACCAATGACCAATCTTG
TTTTAAATGCATGCTCTCAATTGTAAATTTACATGCCCCATGCTAAAACAAGATTGTAAA
ACGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 65-K110679-0022-269-F02C-8409
GenBank Accession LN511426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K110679-0022-269-F02C-8409
CGGACTGAGCCATTTACAATTGAATTATCCTGACGTTTTACATCTTGTTTTAGCATGGGG
CATGTAAATTTACAATTGAGAGCATGCATTTAAAACAAGATTGGTCATTGGTAAAAGCGC
TAAACAGAATACTACCAATCTGATGGTCCTCCCAAGCTTCTTGCTCTGCAAAGGGGTTCA
TTTTCTCTGCGGAACCCATGTCCCTAATCAAATAAGATAATTTAGAACATACGTTAGCCT
TTTACAACACAAAGAAGAGTGATCTTTAAAAGTGTAAGCAAAGTAGAATGTTATGGATTC
CAAGTCATGCATTCTTTCTCATCTCACCTGTACCGTTGTACAGAAACAGCAAACCTCTTC
TCCTGATCAACACTTCCCTGTTGATCATATGCATCCGGCATTCTATACTGTACTCCAACG
AGGTTGAAAAAAAAGAGATAAGTACAAATAATTCAGAAGAAGGAAAACTAAAATCATCAA
GAACACTTGAAGCATTCTAAAAAATAGCCAAAAAGTTAAGAAGGGTACAGTAAGATGCAA
TTACCTCTCCTACATTATCTTCTTCTTGTGTACTTTTCTTTATGAGCTCATAGATTTCTC
TCTTGTATCTGTCAAGATATCAATTCGACCA

Last Updated on 06.12.2018 16:11