Genotyping and primer details


Genotyping and primer details for 269F02 - At2g35340
Line ID 269F02
Locus At2g35340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14875574 of line 269F02 Chr2:14875574
   
Insertion allele amplicon 8474 - 77cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 77cs: 5'-TTGGTCGATCTGATATCTTGACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14875021
   
wt amplicon (over InsPos) 02fg - 77cs
wt amplicon start Chr2:14875021
wt amplicon end Chr2:14875949
wt amplicon size 929 bp
Locus specific primer 77cs: 5'-TTGGTCGATCTGATATCTTGACAG-3'
2nd locus specific primer 02fg: 5'-AGCATGCATTTCATCTTCATACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11