Sequences from confirmation amplicon


Confirmation sequences for line 269G01 and hit At4g16110 are shown.
Line ID 269G01
Locus At4g16110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9113053
Primers used for PCR MI10 - 8474, show primer details
   
Sequence Name 30-K018512-022-269-G01C-MI10
GenBank Accession LN499522 [GenBank]
Sequencing primer gene specific primer, MI10: 5'-CCTCAAAAGGAGAATCTTTAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K018512-022-269-G01C-MI10
AACAGAGAACTAAGCGATGTATATATATCTGAATCTAACAGTGATTCATTCAACAGTCAA
TGAAAACTTCTAGTCAATTCCATAAGCTCTGTTTACTATTTTTGGCAGAGTCAACCACCG
TAATTAAAATCCATAGTTAACCACAAAAATTACAATAAATTTTCACAAATTGGATCATAA
TTCAACATGAAACCTTTGAAATTAATAAAATTTTAGATTAATATTAAAACGAAATTTTGA
TTGAATTTTACCTCTGTAGAGACAAGTCATAAGCATCCTCTCTAAGATCATGAGACAAGT
TGGATCATCATCAACGACAAGAACTCGAAGATTCGCAATGAGTATTCAACGACAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCA
   
Sequence Name 31-K018512-022-269-G01C-8409
GenBank Accession LN499956 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K018512-022-269-G01C-8409
AAAGCCATTTACAATTGAATATATCCTGTCGTTGAATACTCATTGCGAATCTTCGAGTTC
TTGTCGTTGATGATGATCCAACTTGTCTCATGATCTTAGAGAGGATGCTTATGACTTGTC
TCTACAGAGGTAAAATTCAATCAAAATTTCGTTTTAATATTAATCTAAAATTTTATTAAT
TTCAAAGGTTTCATGTTGAATTATGATCCAATTTGTGAAAATTTATTGTAATTTTTGTGG
TTAACTATGGATTTTAATTACGGTGGTTGACTCTGCCAAAAATAGTAAACAGAGCTTATG
GAATTGACTAGAAGTTTTCATTGACTGTTGAATGAATCACTGTTAGATTCAGATATATAT
ACATCGCTTAGTTCTCTGTTCTGTTCTAATATTGTGATCTGCTTAAAGATTCTCCTTTTT
G

Last Updated on 06.12.2018 16:11