Genotyping and primer details


Genotyping and primer details for 269G01 - At4g16110
Line ID 269G01
Locus At4g16110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9113053 of line 269G01 Chr4:9113053
   
Insertion allele amplicon 8474 - MI10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MI10: 5'-CCTCAAAAGGAGAATCTTTAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9113434
   
wt amplicon (over InsPos) 10fq - MI10
wt amplicon start Chr4:9112698
wt amplicon end Chr4:9113434
wt amplicon size 737 bp
Locus specific primer MI10: 5'-CCTCAAAAGGAGAATCTTTAAGCA-3'
2nd locus specific primer 10fq: 5'-GAAATGTAACAGAGAAATCCGAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11