Sequences from confirmation amplicon


Confirmation sequences for line 270A07 and hit At1g33930 are shown.
Line ID 270A07
Locus At1g33930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12326745
Primers used for PCR 13st - 8474, show primer details
   
Sequence Name 22-K116610-0022-270-A07C-13st
GenBank Accession LN496273 [GenBank]
Sequencing primer gene specific primer, 13st: 5'-ATTCACTTGTGGTGATATGTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116610-0022-270-A07C-13st
GTTGCCCTGAATTTCTAAAGGTATGAGTTTGATTATTTGTCCAATGGTTCCTCCTGTCTC
TCATTAATTTATGTTTTTGGATTGGATTGTTCAATTTCAGAATGTTCTCAGACTATGTGG
AGGCAGAAGGGTTGTATTTGATAACCGAACGAAAGATGAAGGTGTGAAGGCTAAGCAAGT
CCAGCAGCTTCTGGTCCATGTCGCAGCGATTGAAAAGGAAACTGGTGGGAATCCCTTTAC
GGACACGATGCACCGTAGGATACAGGTGGAACTATAGTTGATTTGATTGATGCTTCCCAT
ATAAATCATCACTCTCTTGTCTTTGGCCAATAGAAAACTTGTTAATGCTTCATAAATTAC
GGTTTGTCAGGAAGAGGCTGCAAGGGTTAAAAGGGAAGAGAAGGAAATTGAAGAAAAAAA
CATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGATTATTA
   
Sequence Name 06-K116609-0022-270-A07C-8409
GenBank Accession LN488978 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116609-0022-270-A07C-8409
CATTTCATGTTTTTTTCTTCAATTTCCTTCTCTTCCCTTTTACCCTTGCAGCCTCTTCCT
GACAAACCGTAATTTATGAAGCATTAACAAGTTTTCTATTGGCCAAAGACAAGAGAGTGA
TGATTTATATGGGAAGCATCAATCAAATCAACTATAGTTCCACCTGTATCCTACGGTGCA
TCGTGTCCGTAAAGGGATTCCCACCAGTTTCCTTTTCAATCGCTGCGACATGGACCAGAA
GCTGCTGGACTTGCTTAGCCTTCACACCTTCATCTTTCGTTCGGTTATCAAATACAACCC
TTCTGCCTCCACATAGTCTGAGAACATTCTGAAATTGAACAATCCAATCCAAAAACATAA
ATTAATGAGAGACAGGAGGAACCATTGGACAAATAATCAAACTCATACCTTTAGAAATTC
AGGGCAACCATTGGACAAATAATCTTCTAAAGTCATATTTCGATCTTCCAACATATCACA
AAAAGTGAAT

Last Updated on 06.12.2018 16:11