Sequences from confirmation amplicon


Confirmation sequences for line 270A10 and hit At1g09490 are shown.
Line ID 270A10
Locus At1g09490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3065458
Primers used for PCR 81yq - 8474, show primer details
   
Sequence Name 32-K122488-0022-270-A10C-81yq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 81yq: 5'-CAACTACTGGTTAAGTGTTGCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K122488-0022-270-A10C-81yq
GATAGGGTGGATTACGTGGTTCAGTCTTGATCATTGAATCCATTACCATTGGATTACTGG
AGTTTGAATTCATATGGTCATAATGTCATATGACCACTCGTTTATTCCTTGTACCAATTG
ACTTGACGACATCATCACCTAGCGTTACAAGTAAACGTTAAGTGATATTATTAGCTTAGT
TTAGACACTATGTATCCAAACCTTAATTAGTGACAAATTTAGCCTAAACAATAATTATAT
ATTATTGTTTATAAAATGCTTAGATTTTAAAGAGTCTCAACAACTTAAACCACTTGAATT
AGTAATACTAACAAAATAAAAATACACATAATAGTTACAAATATACGAACAGAATCATAA
AAGACATTTCTCCTTGAGGCTAACAATAGTGTCTCTAAGGCTTGATTTCATAGGAGTGAA
CTCTACTCCCAAATTTTTCACTTTCTCCACACACACTTGGCATATCATCTCATTCATCTC
ACTTTCTTCATTCCTAACAAAATCATCAACAAGTAAGTTTTTCAAAATCTTTCATTAAAT
TTATAAACTTCATAAGATGATAAGCAAAGGTAGAGCTAGGGAAAGAGATCTCACGTATCA
GCAATAGACAAATCTGGGAACAGCTTCCGAAGAATGTCTATAATGTCGTTTACACTCATA
TTTGGACCATCGATGATGTATCTGCCATTGGCTGAAGGAGTCTCCAACGCTTTGATATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
   
Sequence Name 01-K122487-0022-270-A10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K122487-0022-270-A10C-8409
CAAGCGTTGGAGACTCCTTCAGCCAATGGCAGATACATCATCGATGGTCCAAATATGAGT
GTAAACGACATTATAGACATTCTTCGGAAGCTGTTCCCAGATTTGTCTATTGCTGATACG
TGAGATCTCTTTCCCTAGCTCTACCTTTGCTTATCATCTTATGAAGTTTATAAATTTAAT
GAAAGATTTTGAAAAACTTACTTGTTGATGATTTTGTTAGGAATGAAGAAAGTGAGATGA
ATGAGATGATATGCCAAGTGTGTGTGGAGAAAGTGAAAAATTTGGGAGTAGAGTTCACTC
CTATGAAATCAAGCCTTAGAGACACTATTGTTAGCCTCAAGGAGAAATGTCTTTTATGAT
TCTGTTCGTATATTTGTAACTATTATGTGTATTTTTATTTTGTTAGTATTACTAATTCAA
GTGGTTTAAGTTGTTGAGACTCTTTAAAATCTAAGCATTTTATAAACAATAATATATAAT
TATTGTTTAGGCTAAATTTGTCACTAATTAAGGTTTGGATACATAGTGTCTAAACTAAGC
TAATAATATCACTTAACGTTTACTTGTAACGCTAGGTGATGATGTCGTCAAGTCAATTGG
TACAAGGAATAAACGAGTGGTCATATGACATTATGACCATATGAATTCAAACTCCAGTAA
TCCAATGGTAATTGGATTCAATGATCAAGACTTGAACCACGTAATCCACCCTTATCCTTA
GAAGCTCATAAATATCACTAAAGGGACAGGCAACACTTA

Last Updated on 06.12.2018 16:11