Sequences from confirmation amplicon


Confirmation sequences for line 270F06 and hit At4g34980 are shown.
Line ID 270F06
Locus At4g34980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16657901
Primers used for PCR CR21 - 8474, show primer details
   
Sequence Name 32-K017404-022-270-F06C-CR21
GenBank Accession LN500373 [GenBank]
Sequencing primer gene specific primer, CR21: 5'-GTTATATCGATCTCAATCGGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K017404-022-270-F06C-CR21
GGATTACTTCGCCGTATTACCTCGATCCAATCGCTATAGGCTCGTACGGCGCCGCGTCGA
AAGGAATCTTCGTCTCTTCCTCTGCCGGAAACGAAGGACCTAACGGTATGTCAGTTACGA
ACCTCGCGCCGTGGGTAACCACCGTTGGTGCTAGTACAATCGATCGGAATTTCCCAGCGG
ATGCTATTCTCGGCGACGGACATCGTCTCAGAGGAGTGTCTCTTTACGCTGGAGTACCTT
TAAACGGTCGTATGTTTCCGGTGGTTTATCCCGGTAAATCGGGAATGTCATCAGCGTCTC
TATGTATGGAGAACACGCTTGATCCGAAGCAAGTGAGGGGTAAAATAGTAATCTGCGATA
GAGGAAGCAGTCCACGCGTAGCCAAAGGATTGGTTGTGAAGAAAGCAGGTGGTGTCGGAA
TGATTCTCGCTAATGGAGCATCTAACGGTGAAGGATTAGTCGGAGATGCTCATCTTATTC
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 34-K017404-022-270-F06C-8409
GenBank Accession LN501217 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017404-022-270-F06C-8409
GTGGAATAAGATGAGCATCTCCGACTAATCCTTCACCGTTAGATGCTCCATTAGCGAGAA
TCATTCCGACACCACCTGCTTTCTTCACAACCAATCCTTTGGCTACGCGTGGACTGCTTC
CTCTATCGCAGATTACTATTTTACCCCTCACTTGCTTCGGATCAAGCGTGTTCTCCATAC
ATAGAGACGCTGATGACATTCCCGATTTACCGGGATAAACCACCGGAAACATACGACCGT
TTAAAGGTACTCCAGCGTAAAGAGACACTCCTCTGAGACGATGTCCGTCGCCGAGAATAG
CATCCGCTGGGAAATTCCGATCGATTGTACTAGCACCAACGGTGGTTACCCACGGCGCGA
GGTTCGTAACTGACATACCGTTAGGTCCTTCGTTTCCGGCAGAGGAAGAGACGAAGATTC
CTTTCGACGCGGCGCCGTACGAGCCTATAGCGATTGGATCGAGGTAATACGGCGAAGTAA
TCCCGTCTCCACCACCGATTGAGA

Last Updated on 06.12.2018 16:11