Sequences from confirmation amplicon


Confirmation sequences for line 271C08 and hit At3g20200 are shown.
Line ID 271C08
Locus At3g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7049233
Primers used for PCR CQ15 - 8474, show primer details
   
Sequence Name 48-K105706-0022-271-C08C-CQ15
GenBank Accession LN506508 [GenBank]
Sequencing primer gene specific primer, CQ15: 5'-GCAGCTTTATGTGCTCTCTCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K105706-0022-271-C08C-CQ15
TACTGTCCTTCTTGCAACACCCTTCCTCTTCATTTTCTGACGTTGGAGCTCCTTCGCCTT
TGATCCATAAGTAAAATTTCTTTCAGTCAGCACTGTACAAGAATATATATACACAGGGAC
GGTTTCGTCTTATATCGTTTTACCTCTTGCTTTGCAGCTAGTGCTTCTCTGCAAGCTGAT
CCATACATGTCAATTGCGTGTTTCAGTTCCTGCTTCAATCTCCGCATCTCAGCTTCCACT
TCTTCCTATACATTTTTCAAGAAAAATATATAGTAAACCATTCCCGAAAACATATGTATT
GCTATCTATAACCAATCAAACGCATCTTACCAAGTTTTGTGGTGAACAAGAACAAGATGA
CCGACCGCTATCTTGTGAGACGAATGAGAAATCATGTTGGATATTTGTGTCATTGAACCT
GATTCCTAGACGCATAGAGTGTCCAGAGCTCGTCGACTCTCTCGGACAGGCTGGTGGTGG
TGGCTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCC
   
Sequence Name 64-K105707-0022-271-C08C-8409
GenBank Accession LN511204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K105707-0022-271-C08C-8409
GAGCCATTTACATTGAATATATCCTGGAGCCACCACCACCAGCCTGTCCGAGAGAGTCGA
CGAGCTCTGGACACTCTATGCGTCTAGGAATCAGGTTCAATGACACAAATATCCAACATG
ATTTCTCATTCGTCTCACAAGATAGCGGTCGGTCATCTTGTTCTTGTTCACCACAAAACT
TGGTAAGATGCGTTTGATTGGTTATAGATAGCAATACATATGTTTTCGGGAATGGTTTAC
TATATATTTTTCTTGAAAAATGTATAGGAAGAAGTGGAAGCTGAGATGCGGAGATTGAAG
CAGGAACTGAAACACGCAATTGACATGTATGGATCAGCTTGCAGAGAAGCACTAGCTGCA
AAGCAAGAGGTAAAACGATATAAGACGAAACCGTCCCTGTGTATATATATTCTTGTACAG
TGCTGACTGAAAGAAATTTTACTTATGGATCAAAGGCGAAGGAGCTCCAACGTCAGAAAA
TTGAAGAGGAAGGGTGGGTGCAAGAAGGACAGTTATCAGAGAAATCTACAAAGTCCATAG
TGGAAAAAGAGAGA

Last Updated on 06.12.2018 16:11