Sequences from confirmation amplicon


Confirmation sequences for line 271D07 and hit At4g31520 are shown.
Line ID 271D07
Locus At4g31520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15280772
Primers used for PCR 30xy - 8474, show primer details
   
Sequence Name 25-K119596-0022-271-D07C-30xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 30xy: 5'-AGTTAAGTCCCTCCCTAGGTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119596-0022-271-D07C-30xy
TTATTCACTATTTGCTTGAACAGTGGCTCCACGGCATCCGAAGGTACCTGCTCGATATAA
AAATGATTGGAAGGATTGCAGGATGAGAAAAGGGATGATGCTTTAAAGTGTAAGATGTTT
GTATACCCCATCATGGCAAGCCTGAACTGCTGCTGCAAGTATTTGTGTAATGTCCTTTAC
ACCATCCTGCATTTAAGAGCATCCCTGTTTCAATAAATTTTCAGCAAAGAAATTGTAAAA
AGGGCAGTTAGCAAATCTTACCTTAGCATACGCTTGAAGATAAGTATAAAATTTGAATAA
AAGCAACTTGTGAAGACCAATTGTTCGTGCAATAACTTTTATAATCATCAACCTGGTCTG
CAAAATTTATTGAATACATGGTTAGTTAGCTCATATGTTTCTATAGAATGGATGAAGCGA
GTTAAAATACAATTACCTCGCGCCGTTCATAAGTTTCCCCAGTGCTCTTGTCATCAGTTT
CCCCACTGCTCTTGTCATTAGTTTCCACAGTGCTCTCGTCAGGCTTAGGCCTAATAAGGG
GAAGCAACCTCTCTGCAAAATTCTGTAAAAGAAGAAAAACATTCAAGTACATAGCCACAT
AGAAAAAGCTAGGAAATGACCACTACAAAAAAGCTAGGAAATGAACCTGTGCATCATTTA
GATGATTAAGAGGTGAATAAGTTGAAGTAGTGTTCTCAGACGACGCACGCTGCTTTCGTT
TAATACTTCGCATGGCGCGTTGCAGTTTTGCTTGCTTTTTCTTCTTGCTAGAAGATGTAC
CTTTGTTGTTGGCCTAAACATCGGAAAATACAAGGAATACCAACTATAATACAAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAGAGTAATAC
   
Sequence Name 01-K119595-0022-271-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119595-0022-271-D07C-8409
TTGAATATATCCTTGTATTATAGTTGGTATTCCTTGTATTTTCCGATGTTTAGGCCAACA
ACAAAGGTACATCTTCTAGCAAGAAGAAAAAGCAAGCAAAACTGCAACGCGCCATGCGAA
GTATTAAACGAAAGCAGCGTGCGTCGTCTGAGAACACTACTTCAACTTATTCACCTCTTA
ATCATCTAAATGATGCACAGGTTCATTTCCTAGCTTTTTTGTAGTGGTCATTTCCTAGCT
TTTTCTATGTGGCTATGTACTTGAATGTTTTTCTTCTTTTACAGAATTTTGCAGAGAGGT
TGCTTCCCCTTATTAGGCCTAAGCCTGACGAGAGCACTGTGGAAACTAATGACAAGAGCA
GTGGGGAAACTGATGACAAGAGCACTGGGGAAACTTATGAACGGCGCGAGGTAATTGTAT
TTTAACTCGCTTCATCCATTCTATAGAAACATATGAGCTAACTAACCATGTATTCAATAA
ATTTTGCAGACCAGGTTGATGATTATAAAAGTTATTGCACGAACAATTGGTCTTCACAAG
TTGCTTTTATTCAAATTTTATACTTATCTTCAAGCGTATGCTAAGGTAAGATTTGCTAAC
TGCCCTTTTTACAATTTCTTTGCTGAAAATTTATTGAAACAGGGATGCTCTTAAATGCAG
GATGGTGTAAAGGACATTACACAAATACTTGCAGCAGCAGTTCAGGCTTGCCATGATGGG
GTATACAAACATCTTACACTTTAAAGCATCATCCCTTTTCTCATCCTGCAATCCTTCCAA
TCATTTTTATATCGAGCAGGTACCTTCGGATGCCGTGGAGCCACTGTTCAAGCAAATAGT
GAATAAGTTTTTACACGATCGCTCAAGTCCGGAGGTGTGATTTCAAACACTA

Last Updated on 06.12.2018 16:11