Sequences from confirmation amplicon


Confirmation sequences for line 271D08 and hit At3g18830 are shown.
Line ID 271D08
Locus At3g18830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6489023
Primers used for PCR NS06 - 8474, show primer details
   
Sequence Name 14-K020068-022-271-D08C-8409
GenBank Accession LN492305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K020068-022-271-D08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGTACATTAAGCCATGTACCTTCCACTGC
TTGTTTGATCCAATCTCAACCTCGGGATTCGGTACCGTCTTCTCGGGGTTACCCTTAGGC
TTACTCTTGGAATCTCTCCACCTGAAACCACTGAAAAGCTCATCCATATCCTCAAGCATC
CTCCCTTGCGTCTCCGGCAAGAAAGTGTAAAAGAAAACCCAAGCCACAGTCGCAATCCCT
CCAAACAAGTAAAACGCTCCACCAGTAGTCATCGCTTTCGACATTGGAAGAAACGATATC
GAGATCACACCACTAGTCACTCTGTTCACCACCACACCCATACTCGAGCCCTGTGATCTC
AACCTCAACGGGAATATCTCCGAGCTATAAACCCAAGTTATCGGTCCAGCTCCTATCGAA
AACGTAGCTACATAAGTCATCACCGTAGCTATAGCTACAACCACAGCCCACATCACTTTC
TTCTCTGACTGGTCTATAATCGTAAGAAGCCCTAACGCTGCTA
   
Sequence Name 13-K020068-022-271-D08C-NS06
GenBank Accession LN491836 [GenBank]
Sequencing primer gene specific primer, NS06: 5'-GGAATGGTACTATCTCTAGCAGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K020068-022-271-D08C-NS06
TTCATAGACCAGTCAGAGAAGAAAGTGATGTGGGCTGTGGTTGTAGCTATAGCTACGGTG
ATGACTTATGTAGCTACGTTTTCGATAGGAGCTGGACCGATAACTTGGGTTTATAGCTCG
GAGATATTCCCGTTGAGGTTGAGATCACAGGGCTCGAGTATGGGTGTGGTGGTGAACAGA
GTGACTAGTGGTGTGATCTCGATATCGTTTCTTCCAATGTCGAAAGCGATGACTACTGGT
GGAGCGTTTTACTTGTTTGGAGGGATTGCGACTGTGGCTTGGGTTTTCTTTTACACTTTC
TTGCCGGAGACGCAAGGGAGGATGCTTGAGGATATGGATGAGCTTTTCAGTGGTTTCAGG
TGGAGAGATTCCAAGAGTAAGCCTAAGGGTAACCCCGAGAAGACGGTACCGAATCCCGAG
GTTGAGATTGGATCAAACAAGCAGTGGAAGGTACATGGCTTAATGTACAATTGTAAATGG
CTTCATGCCGGGAAATCTACTGGATCGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT

Last Updated on 06.12.2018 16:11