Sequences from confirmation amplicon


Confirmation sequences for line 271D11 and hit At3g60100 are shown.
Line ID 271D11
Locus At3g60100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22194779
Primers used for PCR PA59 - 8474, show primer details
   
Sequence Name 31-K037017-022-271-D11C-8409
GenBank Accession LN500071 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K037017-022-271-D11C-8409
CATTTTACTCTTTCTTTTTCAACATGTCTTGTATATAATGATGATCTTCCTTTATTTTAC
CAACTCTTACCAAATTTCTATAGCATGATCAAGGTTTTTTTTTCCCTTGTCTACTGGTTT
CTGTTTTAGTGATCATGAAGGTGGAAATGTTAGTGCTCACGCTGGTCACCTGGTATGTAT
TTGCCACATGATCTTGATGCATGATAATGTTTTGAACTTATAGACATTTCTGATGGTTCT
GTTTTTGGCTAATGATGTTTCTTCACTTAGGTTGGGAGTGCACTTTCAGATCCATATCTT
TCATTTGCTGCTGCATTGAATGGTTTAGCTGGGCCACTCCATGGTTTGGCTAATCAGGTA
TTTATATCCTTAATGAGTCTTATATTGTTCGAATCTACAAGTGTATATAACTTGAACTCT
AAGATTGACTCTTAATTATAAGCTTAAACTTGGAATCTTACTCTGTTTTTTTTCGTTGTC
TTGTTGTAGGAAGTTTTGCTGTGGATCAAATTAGTTGTTGAGGAATGTGGAGAAAGTATA
TCAAAGGAACAGCTAAAAGATTATGTCTGGAAAACGTTAAACAGTGGCAAGGTTGACAA
   
Sequence Name 32-K037017-022-271-D11C-PA59
GenBank Accession LN500494 [GenBank]
Sequencing primer gene specific primer, PA59: 5'-AGCAGAGCATAATTTTGTCAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K037017-022-271-D11C-PA59
TAATCTTTTAGCTGTTCCTTTGATATACTTTCTCCACATTCCTCAACACCTTATTTGATC
CACAGCAAAACTTCCTACAACAAGACAACCATAAAAAACAGAGTAAGATTCCAAGTTTAA
GCTTATAATTAAGAGTCAATCTTAGAGTTCAAGTTATATACACTTGTAGATTCGAACAAT
ATAAGACTCATTAAGGATATAAATACCTGATTAGCCAAACCATGGAGTGGCCCAGCTAAA
CCATTCAATGCAGCAGCAAATGAAAGATATGGATCTGAAAGTGCACTCCCAACCTAAGTG
AAGAAACATCATTAGCCAAAAACAGAACCATCAGAAATGTCTATAAGTTCAAAACATTAT
CATGCATCAAGATCATGTGGCAAATACATACCAGGTGACCAGCGTGAGCACTAACATTTC
CACCTTCATGATCACTAAAACAGAAACCAGTAGACAAGGGAAAAAAAAACCTTGATCATG
CTATAGAAATTTGGTAAGAGTTGGTAAAATAAAGGAAGATCATCATTATATACAAGACAT
GTTGAAAAAGAAAGAGTAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11