SimpleSearch - Line and FST details
Line specific information
| Line ID | 271D11 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N425967) |
| Segregation Analysis | 100:86:65 |
| Confirmed for Hit | At4g18670 At3g60100 |
| Parent of DUPLO pair | 2507 |
| Parent of pair(s) | 9729 |
| Note |
Gene hit At4g18670
| Sequence (A. th genome BLAST matches underlined) | >05-K020163-022-271-D11t-8409 TGAAGCCATTACAATTGAATATATCCTGTGCATAACTGTTCCTTCAACTACGCCATCTCC TGGTTCTCCTCCTACATCGCCGACGACACCTACTCCTGGTGGTTCTCCTCCATCATCACC GACGACACCAACTCCTGGTGGTTCTCCTCCATCATCGCCGACGACACCTACTCCTGGTGG TTCTCCTCCATCATCGCCGACAACACCTTCTCCCGGTGGTTCTCCTCCTTCACCGTCTAT TTCTCCTATGGGATCCTCCCTATAGTGAGAGNNNANGNNGANCCAGTATTTACCAATTTT TTTTCAATTCAAAAATGAAAATGTCCGCAGCATAATAAAACCCNCCCTNTGGGATCCTCC CAATGACAGGCGNNNNNNNCNCNNGNCNNNNNTNAAGCAAGAAAACCCCCNGNANTACGN GNCGCCANNNATAATNAANAAGAAACCCGGANNGNNNNNCTTTTTTTTTTTTACACCCCC CCCCCANTGGGTTTTTTCNNNATTTAAAAAAAAAAACGAGGGNATGGNTAATTTAAGGGA ACANTTTTACAGAAANATTTTCCATTTTATATGCTTTTACCGGGAGATCTCGGGGATAAC CNTGAGTATAATGGTCAATATGGGAAAAAAAAAAAAGAANAATTCACATTTTTTTTCATT CAAAATGTACATTCCCCCCCNTTATTATAA |
| GenBank Accession | BX530852 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:10277128 - go to primer design |
| BLAST e Value | 5e-112 |
| Hit Clone Code (BAC ID) | F28A21 |
| Hit Gene Code | At4g18670 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Leucine-rich repeat (LRR) family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g22750
| Sequence (A. th genome BLAST matches underlined) | >84-K015071-022-271-D11-8409 TGATCATGAGATTTCCNGGACATGAAGCATTTACATTGAATATATCCTGATCCATGTATA ATTTCCCGTGTGGAACATATCGACTATTTTTGATAATCTATTGCCATTCACCCCTTAGGG AGACTCCCTATAGTGAGACCGGGCAAGTTTCTCCCTCCTCGAGAAAACAGATGCCCCCCA TCTCAAACCTCCCAGTTGCTCCGTATGCATGGTGCTGCGTTTCCTCCCTTGCATAGATGA GGACCCCTACCATGCCCAGATTCCCCATGGTTCAATCCTCAAAAACCGTACAATGCTGTA TAAATCCTCCAGATTGTTCAGTCAATAAGATAACATGTGAAATGATCACAAAGGTCCAAA TAGGACAAAAAATTACTATTCTCCATCCATTTGAATGAACCAAGATATCATGCCAGCGGA CATATGGATACCAAAATTGCAAGCCATATGAAATAAATCTTCAAAGACAACTTTGTGAGA TAACGACGAGACTTGTGTACCATAAATCATGCACAACAAGTTGATATCCTTCTAACAGAG ATTTAGACCATTCGAAACTCAGGTGAATAACAAATGTCCAACTACTAAAATTCTTCCATA CATAGAATGGCAGTGTCCTAATAAAGTTTGGCCTATAGGACCGGCCACAACCAAGATTTT CTTTCCAAACCCTATGGGATCT |
| GenBank Accession | CT026218 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:7567526 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | MDJ22 |
| Hit Gene Code | At5g22750 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | DNA/RNA helicase protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At3g60100
| Sequence (A. th genome BLAST matches underlined) | >05-K020162-022-271-D11t-8409 ACGGATTCAGAGGCTCTCGGCAACCAAGGAGGAGCAACGCATGCTAATACCAACGACAAC CAGATTTCTATAGCAACATCACGGTTATCTTTGTCCCCAGTGTACGGTCTCTCATAGTTA TGAGAAGATGAAGGTGAAAATGTTAGTGCTCACGCTGTTCACCTGGAATGTATTTGCCCC TTGATCTTAATGCATGATAATGTTTTGAATTTATAGACATTTCTGATGGTTTTGTTTTTG GCTAATGATGTTTCTTCACTTAGGTTGGGAGTGCACTTTCAAATCCATATCTTTCATTTG CTGCTGCATTGAATGGTTTAGCTGGGCCACTCTATGGTTTGCTTAATAAACTATGGGATC CTCCCTATAGTGAG |
| GenBank Accession | BX530851 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:22194772 - go to primer design |
| BLAST e Value | 1e-93 |
| Hit Clone Code (BAC ID) | T2O9 |
| Hit Gene Code | At3g60100 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | citrate synthase 5 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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