Sequences from confirmation amplicon


Confirmation sequences for line 271D11 and hit At4g18670 are shown.
Line ID 271D11
Locus At4g18670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10277118
Primers used for PCR PA60 - 8474, show primer details
   
Sequence Name 33-K037018-022-271-D11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K037018-022-271-D11C-8409
AGCCATTTACATTGAATATATCCTGTGCATAACTGTTCCTTCAA
   
Sequence Name 34-K037018-022-271-D11C-PA60
GenBank Accession LN501327 [GenBank]
Sequencing primer gene specific primer, PA60: 5'-AGAGTAAACCGGAGTAGGTGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K037018-022-271-D11C-PA60
AGAAGGTGTTGTCGGCGATGATGGAGGAGAACCACCAGGAGTAAGTGTCGTCGGCGATGA
TGGAGGAGAACCACCAGGAGTTGGTGTCGTCGGCGATGATGGAGGAGAATTTTTTTTAGT
AGGTGTCGTCGGCGATGTAGGAGGAGAACCAGGAGATGGAGTAGTTGAAGGAACAGTTAT
GCACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11