Sequences from confirmation amplicon


Confirmation sequences for line 271F06 and hit At5g43090 are shown.
Line ID 271F06
Locus At5g43090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17297160
Primers used for PCR CQ16 - 8474, show primer details
   
Sequence Name 03-K105916-0022-271-F06C-CQ16
GenBank Accession LN487490 [GenBank]
Sequencing primer gene specific primer, CQ16: 5'-ACTCGGCACATTTCTAAGTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105916-0022-271-F06C-CQ16
GATGGGTACCCTTCTATCATTATTCTTTACTTTGAGATATGATTGTGCATTGTTTCCTTG
GATCTTGATTTTCTATAATTCTTTCTTTTGGAGATATCAGAACTTTGGCAATACAAGTTC
TGTTAACTAGTATTCATGAGCGTGCTAACAATCAAATCCCGCGCATTATGGATGCTATAA
GCTCGGTAGCACTTCAATTGACAAGGAACACCAATGCCAAATATGTGATTCTAGCATGTT
TCCGCATGTTTACTTCGTCGCAGTGTAGGGTAAGCATCTCATTCACATATTAATCTACTT
CAGTTCTCAAAACCTATATAGAATATTTGTTTCTTGTCAGAGACAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K105917-0022-271-F06C-8409
GenBank Accession LN487491 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105917-0022-271-F06C-8409
TTACAATTGTCTCTGACAAGAAACAAATATTCTATATAGGTTTTGAGAACTGAAGTAGAT
TAATATGTGAATGAGATGCTTACCCTACACTGCGACGAAGTAAACATGCGGAAACATGCT
AGAATCACATATTTGGCATTGGTGTTCCTTGTCAATTGAAGTGCTACCGAGCTTATAGCA
TCCATAATGCGCGGGATTTGATTGTTAGCACGCTCATGAATACTAGTTAACAGAACTTGT
ATTGCCAAAGTTCTGATATCTCCAAAAGAAAGAATTATAGAAAATCAAGATCCAAGGAAA
CAATGCACAATCATATCTCAAAGTTAAAGAATAATGATTAGAAGTGTGTACCCGATCGGA
TTAAAGCATATGTTAACAAACTTAGAAATGTGCCGAG

Last Updated on 06.12.2018 16:11