Sequences from confirmation amplicon


Confirmation sequences for line 271G03 and hit At3g30340 are shown.
Line ID 271G03
Locus At3g30340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11958427
Primers used for PCR 62we - 8474, show primer details
   
Sequence Name 45-K118511-0022-271-G03C-62we
GenBank Accession LN505689 [GenBank]
Sequencing primer gene specific primer, 62we: 5'-TTTGGTCACTGATGCAGACTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118511-0022-271-G03C-62we
CCCACGATATGACCATTGATCCTATCACACNTTTTTGTCATGCCACATATATAGTTCATT
TGTTAGATATTATTAGCAATAAAAGTCAAAAAGAACCATTTAATTAGCTTTACGTGAAAG
AAACCCATCTACTAATTAGCTTTCTCCTATGTAATCATTTCTAATCCCATATTTAATCCG
GTTCAAATTCAGTTATATAACACACAGATTAAAGGTCTATTATGGGTCTCAATGATATTC
TATAAAGCTAACAATTTTTAAAATTGTCTATTGCAATGCATGCATCAATTTGGTGTAAAG
CGTAAAATTCCAATTACACATTTGAATGCAGATGAAAGGGTCTTTAATATAGGCATATTC
TCTACTCTCTTGTACTTACTAGTCAATAGATATTTGAATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATAA
AA
   
Sequence Name 17-K118510-0022-271-G03C-8409
GenBank Accession LN494067 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118510-0022-271-G03C-8409
CATTTACAATTGAATATATTCAAATATCTATTGACTAGTAAGTACAAGAGAGTAGAGAAT
ATGCCTATATTAAAGACCCTTTCATCTGCATTCAAATGTGTAATTGGAATTTTACGCTTT
ACACCAAATTGATGCATGCATTGCAATAGACAATTTTAAAAATTGTTAGCTTTATAGAAT
ATCATTGAGACCCATAATAGACCTTTAATCTGTGTGTTATATAACTGAATTTGAACCGGA
TTAAATATGGGATTAGAAATGATTACATAGGAGAAAGCTAATTAGTAGATGGGTTTCTTT
CACGTAAAGCTAATTAAATGGTTCTTTTTGACTTTTATTGCTAATAATATCTAACAAATG
AACTATATATGTGGCATGACAAAAAGTGTGATAGGATCAATGGTCATTATCGTGGGACTT
TATATACTTTTATGGGGAAAAAGCAAGGATAAGTCTGCATCGTTGACCAA

Last Updated on 06.12.2018 16:11