Sequences from confirmation amplicon


Confirmation sequences for line 272A04 and hit At1g76640 are shown.
Line ID 272A04
Locus At1g76640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28765458
Primers used for PCR JA22 - 8474, show primer details
   
Sequence Name 14-K100937-0022-272-A04C-8409
GenBank Accession LN492429 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K100937-0022-272-A04C-8409
CGGACTGAGCCATTTACATTGAATATATCCGGAGTAATGCAATCTTCTCCATCAGCAATG
TACATTCTAAAAGCTTCCATTATCTTCCTCTTCTTTTCTTCCTCAGTGAATTCATCATTC
CCTTTGATAAGCAGAGCAAACTCATTAATATCCAACATCCCATCACCGTCTATATCAGAG
AGTTTAACCGCAGCCTCGGCTTCTTCATCAGACATTTGCTCTCCCAACGTCTTAAAACTC
TTCTTAAGCTCCTCTGCAGAGATTCTACCGTCTCTGTTTGCATCCATGTAAGCAAACACA
GCCTCTAAGTCTCTGTTCTTCTCCTCTAAGAATTTCATGAAAGATGAAGATAACTGACGT
TGAGTGTTCTTCATTTTGAGAAAGAAAAGATTGTATTTGTTATAAGTTACAAGTATTGTT
CTTTCTTTTTTTGATTTAGGATTTGAATGAGAAATGGTATAAAGTGTTTCTCTATATATA
TAGGTGTGTTTCAAGTCGAGAATAGTAATAAGAAAATAGAAATTGAAACGAATCAAACGT
GTGAGATTCAGATCTAGAAAAAAAAAACAAATATTTTTAGTCTTTAGATCTAGAAGAAGT
AGTATTATTAGTCTTCTAGATCTAGCTGGGAAAGGAAATATTAGTATATTGGTTCGTTAC
ATATCTCATCCCCATGTTTAAAACACGTTTTCTTCTTCTACCTCTCTGTCTTAAATATAG
GAAGAAGGATTGAGAAATGTTATTGTGGTAAATATAATATTTGACGACTCCCATTTGTTG
ATTTTAGAACAATGGTAACTTCATCTACAAATCATTGCTTTTTTTCAAAAATCAATTTCT
TCACAACCTAAGGTTTCATACCAAAGAAGTCCATATA
   
Sequence Name 14-K100943-0022-272-A04C-JA22
GenBank Accession LN492430 [GenBank]
Sequencing primer gene specific primer, JA22: 5'-GCCATAACAAGTTTGAGGCATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K100943-0022-272-A04C-JA22
CTTAGTTGTGAGAAATTGATTTTTGAAAAAAAGCAATGATTAGTAGATGAAGTTACCATT
GTTCTAAATTCAACAAATGGGAGTCGTCAAATATTATATTTACCACAATAACATTTCTCA
ATCCTTCTTCCTATATTTAAGACAGAGAGGTAGAAGAAGAAAACGTGTTTTAAACATGGG
GATGAGATATGTAACGAACCAATATACTAATATTTCCTTTCCTAGCTAGATCTAGAAGAC
TAATAATACTACTTCTTCTAGATCTAAAGACTAAAAATATTTGTTTTTTTTTTCTAGATC
TGAATCTCACACGTTTGATTCGTTTCAATTTCTATTTTCTTATTACTATTCTCGACTTGA
AACACACCTATTTTTATGGAGAAACACTTTATACCATTTCTCATTCAAATCCTAAATCAA
AAAAAGAAAGAACAATACTTGTAACTTATAACAAATACAATCTTTTCTTTCTCAAAATGA
AGAACACTCAACGTCAGTTATCTTCATCTTTCAGGAAATTCTTAGAGGAGAAGAACAGAG
ACTTAGAGGCTGTGTTTGCTTACATGGATGCAAACAGAGACGGTAGAATCTCTGCAGAGG
AGCTTAAGAAGAGTTTTAAGACGTTGGGAGAGCAAATGTCTGATGAAGAAGCCGAGGCTG
CGGTTAAACTCTCTGATATAGACGGTGATGGGATGTTGGATATTAATGAGTTTGCTCTGC
TTATCAAAGGGAATGATGAATTCCCTGAGGAAGAAAAGAAGAGGAAGATAATGGAAGCTT
TTAGAATGTACATTGCTGATGGAGAAGATTGCATTACTCCGGATATATTCAATTGTAAAT
GGCTTCATGTCCG

Last Updated on 06.12.2018 16:11