Sequences from confirmation amplicon


Confirmation sequences for line 272B08 and hit At4g19410 are shown.
Line ID 272B08
Locus At4g19410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10582502
Primers used for PCR 06kq - 8474, show primer details
   
Sequence Name 37-K113817-0022-272-B08C-06kq
GenBank Accession LN502687 [GenBank]
Sequencing primer gene specific primer, 06kq: 5'-TCTCTCTCTTTCTCTCTGGATTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K113817-0022-272-B08C-06kq
TACTCGATTATATACAAGTAAAGATATACTCTTTATTCAATTGCAGTGATCATTATTGAT
CCGATTTGGAAAACAAATCCAAAATGTTTGCGAGCTCTTTCAATTTCAAATTAAAAAAAA
TTGAACATTTAGAGATGATTCTAGTCTTCAGTAGAGATAGCAGGACAAGTAGGATTACAA
GTCGGCGACGGACAATCTATCTTCTGAAATGCACTCCGGCCATAAAACCAGTTTCCGACT
GCTTTCGCAATTCTCTGCAAAAACAAATAATCAAAATCAGAACTAAAATCGAATTGAAAA
GATTTTAAAAGTATTGGAGGATTGAATTTTACCGTATTGGCGACTTGGGGACCTTTGTCA
CCGGACCAAGAGGCGGCGCTTCCTCCTTGGCAATGAGCGTGACACGAGTCTAAGAACAAT
CCTCTAGACGGTGTACTGTGAACCGGTGACAATGCGAGAAATCTACATGGATCAGCAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
   
Sequence Name 11-K113816-0022-272-B08C-8409
GenBank Accession LN491196 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K113816-0022-272-B08C-8409
GATCCATGTAGATTTCTCGCATTGGTCACCGGTTCACAGTACACCGTCTAGAGGATTGTT
CTTAGACTCGTGTCACGCTCATTGCCAAGGAGGAAGCGCCGCCTCTTGGTCCGGTGACAA
AGGTCCCCAAGTCGCCAATACGGTAAAATTCAATCCTCCAATACTTTTAAAATCTTTTCA
ATTCGATTTTAGTTCTGATTTTGATTATTTGTTTTTGCAGAGAATTGCGAAAGCAGTCGG
AAACTGGTTTTATGGCCGGAGTGCATTTCAGAAGATAGATTGTCCGTCGCCGACTTGTAA
TCCTACTTGTCCTGCTATCTCTACTGAAGACTAGAATCATCTCTAAATGTTCAATTTTTT
TTAATTTGAAATTGAAAGAGCTCGCAAACATTTTGGATTTGTTTTCCAAATCGGATCAAT
AATAATCACTGCAATTTGAATAAAGAGTATATCTTTACTTTGTATTATAATCGAGTTTAC
CTATAAAGTTTTTAACTTTTTATACGAATCCAGAGAGAAGAAGAGAGA

Last Updated on 06.12.2018 16:11