Sequences from confirmation amplicon


Confirmation sequences for line 272G09 and hit At3g12020 are shown.
Line ID 272G09
Locus At3g12020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3831310
Primers used for PCR 14kk - 8474, show primer details
   
Sequence Name 69-K113595-0022-272-G09C-14kk
GenBank Accession LN512246 [GenBank]
Sequencing primer gene specific primer, 14kk: 5'-ATACACTGAAATTTGCTCATCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113595-0022-272-G09C-14kk
AACAGGGTTTTCCCGCATATCTTCTTTCAGCTCTTATCTTTTGTACTGAAGGTCTTTTTT
GGTTTCTAACTCTAGAAAACTGTAGATAATTGATGAGAAATCATTAATCAAGAAGTACCA
ACGCGAGATTCGTCAACTGAAGGAAGAGTTGGAACAGCTTAAACAGGAAATTGTACCAGT
TCCTCAGCTGAAGGATATTGGTGCAGATGATATTGTTCTTCTGAAGCAGAAGGTATGCTT
GAAATTTTTGTTCGGCACTGATGTTTTCTGAGAACAATTTGTCAGAAAGAGATTATGGCA
GATAGAGGTCAAATTTGATAGAGGTCATTCTGGACACAAAATGCGACAGTTTCTCCATAT
TGGTAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCA
   
Sequence Name 27-K113594-0022-272-G09C-8409
GenBank Accession LN498474 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113594-0022-272-G09C-8409
TCATTGAATATATTACCATATGGAGAAACTGTCGCATTTTGTGTCCAGAATGACCTCTAT
CAAATTTGACCTCTATCTGCCATAATCTCTTTCTGACAAATTGTTCTCAGAAAACATCAG
TGCCGAACAAAAATTTCAAGCATACCTTCTGCTTCAGAAGAACAATATCATCTGCACCAA
TATCCTTCAGCTGAGGAACTGGTACAATTTCCTGTTTAAGCTGTTCCAACTCTTCCTTCA
GTTGACGAATCTCGCGTTGGTACTTCTTGATTAATGATTTCTCATCAATTATCTACAGTT
TTCTAGAGTTAGAAACCAAAAAAGACCTTCAGTACAAAAGATAAGAGCTGAAAGAAGATA
TGCGTGGAAAACCTTGTTTTGTTCAGCTTGAATCTCAATATGCTTTGCACGATGAGCAAA
TTCCAGTGTA

Last Updated on 06.12.2018 16:11