Sequences from confirmation amplicon


Confirmation sequences for line 272H08 and hit At1g75140 are shown.
Line ID 272H08
Locus At1g75140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28202242
Primers used for PCR 09qw - 8474, show primer details
   
Sequence Name 33-K115837-0022-272-H08C-09qw
GenBank Accession LN501130 [GenBank]
Sequencing primer gene specific primer, 09qw: 5'-GAGCCATCGAGAATCAACTAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115837-0022-272-H08C-09qw
TACTCCTTTATAGCAAGCGACCGCGAAAGCTGCTTGTGATGGGTTTAGGAGACGGATACG
TAGCTACATACAAATCAAAGCTTCCAATCTCCAAAGCAGAATTCAACACAATGCTTTGGA
GCAGTCCTGTCTTCTTCTTCATACTTTTCTTATTCGGCGCTTGGCATTTTTTCTCTAAGA
AGAAAGAATCTCTCACAGCTTGGGGACCTGACGATCCTTTCTCCTCCACGACAATGTCTT
CTTCTTCTACAACAACTGCACAAAATAGTTCCGCATTCTCTGAATCAACTAGGAGAAACG
ACGACCACATGGATCTTCGGAGAAGATACGTTTCGCCATCTCGGTATCCTCCTGGAGCAG
CAACAGGTGCTTACCGTTCAGTAGGGTCTAACGACCCGAGCTCAAGAGCTCCAGTGGAAA
CTACTAACTACAGAACAACCGCACAGGAGATGAAGTATCGTGGTGGGTCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCA
   
Sequence Name 01-K115833-0022-272-H08C-8409
GenBank Accession LN486712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115833-0022-272-H08C-8409
ATTTCATTGAATTATCCTGACCCACCACGATACTTCATCTCCTGTGCGGTTGTTCTGTAG
TTAGTAGTTTCCACTGGAGCTCTTGAGCTCGGGTCGTTAGACCCTACTGAACGGTAAGCA
CCTGTTGCTGCTCCAGGAGGATACCGAGATGGCGAAACGTATCTTCTCCGAAGATCCATG
TGGTCGTCGTTTCTCCTAGTTGATTCAGAGAATGCGGAACTATTTTGTGCAGTTGTTGTA
GAAGAAGAAGACATTGTCGTGGAGGAGAAAGGATCGTCAGGTCCCCAAGCTGTGAGAGAT
TCTTTCTTCTTAGAGAAAAAATGCCAAGCGCCGAATAAGAAAAGTATGAAGAAGAAGACA
GGACTGCTCCAAAGCATTGTGTTGAATTCTGCTTTGGAGATTGGAAGCTTTGATTTGTAT
GTAGCTACGTATCCGTCTCCTAAACCCATCACAAGCAGCTTTTCGCGGTCGCTTGCTATT
AAAGGAGTTACCTTTTCTAGCTTCTGGTGATCTGTGGTTTTAGTTGATCTGNAATGGCT

Last Updated on 06.12.2018 16:11