Sequences from confirmation amplicon


Confirmation sequences for line 273E02 and hit At1g76540 are shown.
Line ID 273E02
Locus At1g76540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28721657
Primers used for PCR 14ns - 8474, show primer details
   
Sequence Name 37-K114649-0022-273-E02C-14ns
GenBank Accession LN502712 [GenBank]
Sequencing primer gene specific primer, 14ns: 5'-ATCTGCTATTTTGAGCCTCATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K114649-0022-273-E02C-14ns
AACAGGGAGAAATAGTAAGAAGAAGAAATCCTCTCTTTCAAGTTATTATCTAAAATCATT
CTCACTTAGAAGTTGAAAACAAGGGAAAGCCAGAGAACAATTCACCTGTGCAATATCCCG
TGACCATGGCAGAATGCCATACCTTTGCATAGTTGATACATCAAGCTCTGCAAATAACTT
TTCAGAATGTTATGTTTGGTTCAAGTGAGCACAAATGAAGCGAGAAACCATATTCAAAGA
CGAAAGAAGAAAAAGAAATGGCTTCATGTCCGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 08-K114648-0022-273-E02C-8409
GenBank Accession LN489835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114648-0022-273-E02C-8409
CCGGACATGAAGCCATTTCTTTTTCTTCTTTCGTCTTTGAATATGGTTTCTCGCTTCATT
TGTGCTCACTTGAACCAAACATAACATTCTGAAAAGTTATTTGCAGAGCTTGATGTATCA
ACTATGCAAAGGTATGGCATTCTGCCATGGTCACGGGATATTGCACAGGTGAATTGTTCT
CTGGCTTTCCCTTGTTTTCAACTTCTAAGTGAGAATGATTTTAGATAATAACTTGGAAGA
GAGATTTCTTCTTCTTACTATTTCTCCTTGTTTCAAAACAGAGATCTCAAGCCTCACAAT
CTCTTGATGGATCCCAAGACAATGAGGCTCNAAATAGCAG

Last Updated on 06.12.2018 16:11