Sequences from confirmation amplicon


Confirmation sequences for line 275D04 and hit At3g21690 are shown.
Line ID 275D04
Locus At3g21690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7641452
Primers used for PCR RU48 - 8474, show primer details
   
Sequence Name 11-K024450-022-275-D04C-8409
GenBank Accession LN490939 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K024450-022-275-D04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAGTGATTCCATTATTTGTCGTTAACCT
TCGCCACAAACGTTTGCCAACCGCATCCAACCGCCACACCTATAATAAAATTTTCCCAAT
TGTTCAAAAAAAAAAAAAAAATCTTCCCTTATTCGGTTTGCAAAATTGCGGGGTATCGAT
CAAAAGGGTTTCCCAAAGAGGACAGGTTGGATTCCGTTAAGGACAAGTGTTCCGGCAAGA
AGCGGACAGAGATCAAAAACAGCGCCCGAAACTTCTTTCCCCTCAGGGAAAGCATACCTT
AAAACATCGCGGCCCGCTAAAATAACAAGGGCTAGGATCCCCCAGGTGATTAACGAATAA
TTGTTACCGATGATCCCAGAA
   
Sequence Name 12-K024450-022-275-D04C-RU48
GenBank Accession LN491398 [GenBank]
Sequencing primer gene specific primer, RU48: 5'-CAGCTTTTTCTGTGATCATCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K024450-022-275-D04C-RU48
ATTCGTTAATCACTTGTGTGATCCTAGCCATTGTTATTTTAGCGTGCCGCGATGTTTTAA
GCTATGCTTTCACTGAGGGTAAAGAAGTCTCGGACGCTGTTTCTGATCTCTGTCCGCTTC
TTGCCGTAACACTTGTCCTTAACGGAATCCAACCTGTCCTCTCTGGTAAACCCTTCTGAT
CGATACACCGCAATTTCGCAAACCGAATAAGCGAAGATTTTTTTTTTTTTTTTGAACAAT
TGGGAAAATTTTTTTAAAGGTGGGGCGGTTGAATGCGGTTGGCAAACTTTTGGGGCAAAG
GTTAACCACAAAAAAGGGAATCCCCCATTTGAAAAGGGCTTCTTGTCCGGAAAATCTACT
TGGATCACCAATGAGTATAAGGGCCAATTTGGAAAAAAAAAAAGAGTATTTCCCATTTTT
TTTTCATTTCAAAAAGGAAAATTCCCCCCCCTTTTTTAAAAAAGAAAATTCCTTTTTGAA
A

Last Updated on 06.12.2018 16:11