Sequences from confirmation amplicon


Confirmation sequences for line 275E02 and hit At3g56770 are shown.
Line ID 275E02
Locus At3g56770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21030200
Primers used for PCR PH68 - 8474, show primer details
   
Sequence Name 17-K017512-022-275-E02C-PH68
GenBank Accession LN493657 [GenBank]
Sequencing primer gene specific primer, PH68: 5'-ATTATGTGCCTCGTCTACTTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K017512-022-275-E02C-PH68
GTCGTCGTCTGAACCCTTTTGCCACGGTTTATGAAGACAAAGCTCTTGCTTCTCTGAGGA
ACCATAAAGAGGCTGAGCGAAAGAGAAGAGCAAGAATCAATTCCCATCTCAACAAGCTCC
GCAAGTTACTCTCTTGTAACTCCAAGGTACACAAATCTTTTACTTTACTATCTCACTACT
CTACTCTAAACTTATAATGAAACTTAGATAGAGACTTGAACCGGAAAATTATAAAAAGCA
TAGAAGATAAAAATGGAAGATTAAGCATGATGAAACTATTAATTCTTTTGGGTTATTTAA
GATATGAAATTAACTTCTAAAGTTACATATTCTTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 21-K017512-022-275-E02C-8409
GenBank Accession LN495457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017512-022-275-E02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAGAATATGTAA
CTTTAGAAGTTAATTTCATATCTTAAATAACCCAAAAGAATTAATAGTTTCATCATGCTT
AATCTTCCATTTTTATCTTCTATGCTTTTTATAATTTTCCGGTTCAAGTCTCTATCTAAG
TTTCATTATAAGTTTAGAGTAGAGTAGTGAGATAGTAAAGTAAAAGATTTGTGTACCTTG
GAGTTACAAGAGAGTAACTTGCGGAGCTTGTTGAGATGGGAATTGATTCTTGCTCTTCTC
TTTCGCTCAGCCTCTTTATGGTTCCTCAGAGAAGCAAGAGCTTTGTCTTCATAAACCGTG
GCAAAAGGGTCAGACGACGACGTCACGGTGGTGGAAGTAGACGAGGCACATA

Last Updated on 06.12.2018 16:11