Sequences from confirmation amplicon


Confirmation sequences for line 277B05 and hit At5g44990 are shown.
Line ID 277B05
Locus At5g44990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18163865
Primers used for PCR CQ19 - 8474, show primer details
   
Sequence Name 67-K106189-0022-277-B05C-CQ19
GenBank Accession LN511819 [GenBank]
Sequencing primer gene specific primer, CQ19: 5'-TACATCAACAATGGCTACACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K106189-0022-277-B05C-CQ19
ACCGCAACTCGTTCCGAACTTTGTTTCAAAGATCCTGACTCTCAGTTTCCAGCAGAATCA
GGAAGATACCATCTTTATATCTCTTATGCTTGTCCATGGGCTTCTAGATGTCTTGCAATC
TTGAAACTCAAAGGACTTGACAAAGCTATAAGCTTTTCGGTTAGTAAAGACGACACATTT
AGTTTCGATTTTTACAGAACATAATCTGATCAGTTTGTTCATTAGTCTGTTCAACCACTT
TGGAGAAATACCAAAGAAAACGACGAGCATATGGGATGGGTTTTTCCAGATTCAGATACA
GAGGTTCTTGGAGCTGAACGTGATCATATCAATGGTGCGAAAAGCGTAAGAGAACTTTAT
GATATCGCTAGCTCCAACTACACGGGCAAATATACGGTTCCTGTAAGTTCTTTTAATCAT
GGTGTGGTCTAATCTTAAGTCTTATTTTGTTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 59-K106190-0022-277-B05C-8409
GenBank Accession LN510008 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K106190-0022-277-B05C-8409
GCCATTTACAATTGAACAAAATAAGACTTAAGATTAGACCACACCATGATTAAAAGAACT
TACAGGAACCGTATATTTGCCCGTGTAGTTGGAGCTAGCGATATCATAAAGTTCTCTTAC
GCTTTTCGCACCATTGATATGATCACGTTCAGCTCCAAGAACCTCTGTATCTGAATCTGG
AAAAACCCATCCCATATGCTCGTCGTTTTCTTTGGTATTTCTCCAAAGTGGTTGAACAGA
CTAATGAACAAACTGATCAGATTATGTTCTGTAAAAATCGAAACTAAATGTGTCGTCTTT
ACTAACCGAAAAGCTTATAGCTTTGTCAAGTCCTTTGAGTTTCAAGATTGCAAGACATCT
AGAAGCCCATGGACAAGCATAAGAGATATAAAGATGGTATCTTCCTGATTCTGCTGGAAA
CTGAGAGTCAGGATCTTTTGAAACAAAGTTTCGGAACGATGTTGCGGTTCTAGCAAAATT
CGGATTTTCGTTTTCCATTGGTGTAGC

Last Updated on 06.12.2018 16:11