Sequences from confirmation amplicon


Confirmation sequences for line 277G04 and hit At3g05790 are shown.
Line ID 277G04
Locus At3g05790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1720244
Primers used for PCR 35zk - 8474, show primer details
   
Sequence Name 63-K128224-0022-277-G04C-35zk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 35zk: 5'-TCAGATTATGTAGGCAAGCCAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K128224-0022-277-G04C-35zk
CGGGGTTGTAATGGGTCTAGCTTGGGACATCCATGGGGGGCTCAACATTGTACATAGAGA
CAACCTTTGTAGAAGAAGGAGAAGGCAAAGGTGGTCTTCATATAACGGGTCGGCTTGGGG
ATGTGATGAAAGAAAGCGCAGAGATAGCTCACACAGTCGCGAGAAGAATAATGCTGGAGA
AAGAACCCGAGAACAAGTTGTTTGCGAATTCCAAGCTTCATCTACATGTACCTGCAGGAG
CCACACCAAAAGACGGCCCAAGCGCAGGCTGCACCATGATAACTTCATTGCTATCACTTG
CCTTGAAGAAGCCTGTAAGGAAGGATCTTGCAATGACTGGAGAAGTCACTTTGACCGGTA
GAATCCTTGCGATTGGCGGCGTAAGTGAAAATTCACACCACAATAACTCTGTTTTAGCTT
CTTAGTTACCAAATTCTTGTATTAAGCAGTGTAATACAGAAAATATCTCTTAATATCGCT
TTGTCAGGTGAAAGAGAAGACTATAGCTGCAAGACGGAGTCAAGTGAAGGTGATAATATT
CCCTGAGGCTAACCGGAGAGATTTTGATGAGCTGGCGAGAAACGTGAAAGAAGTCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 58-K128223-0022-277-G04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K128223-0022-277-G04C-8409
CTCATCAAAATCTCTCCGGTTAGCCTCAGGGAATATTATCACCTTCACTTGACTCCGTCT
TGCAGCTATAGTCTTCTCTTTCACCTGACAAAGCGATATTAAGAGATATTTTCTGTATTA
CACTGCTTAATACAAGAATTTGGTAACTAAGAAGCTAAAACAGAGTTATTGTGGTGTGAA
TTTTCACTTACGCCGCCAATCGCAAGGATTCTACCGGTCAAAGTGACTTCTCCAGTCATT
GCAAGATCCTTCCTTACAGGCTTCTTCAAGGCAAGTGATAGCAATGAAGTTATCATGGTG
CAGCCTGCGCTTGGGCCGTCTTTTGGTGTGGCTCCTGCAGGTACATGTAGATGAAGCTTG
GAATTCGCAAACAACTTGTTCTCGGGTTCTTTCTCCAGCATTATTCTTCTCGCGACTGTG
TGAGCTATCTCTGCGCTTTCTTTCATCACATCCCCAAGCCGACCCGTTATATGAAGACCA
CCTTTGCCTTCTCCTTCTTCTACAAAGGTTGTCTCTATGTACAATGTTGAGCCCCCCATG
GATGTCCAAGCTAGACCCATTACAACCCCGACTGGTGTCTGCTCATATATCTTCTCTTCC
TGGAATACTGGCTTGCCTACATAATCTG

Last Updated on 06.12.2018 16:11