Sequences from confirmation amplicon


Confirmation sequences for line 278C01 and hit At1g48430 are shown.
Line ID 278C01
Locus At1g48430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17905136
Primers used for PCR 08dc - 8474, show primer details
   
Sequence Name 21-K109029-0022-278-C01C-08dc
GenBank Accession LN495694 [GenBank]
Sequencing primer gene specific primer, 08dc: 5'-ATTGTTGGAGATGATTGTGCTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K109029-0022-278-C01C-08dc
TTAGCGGGACAGTTCTTGTCATAAGGTTCTCAGTTTTCTTCTACCTTATAGTTCCTCTAT
CTGCACTATAAGATAGCATTTCTTAGCATTGTTTGCAGTCACTTATCCTCATCTTTTTTT
ATTATTGCATTTTCTTATATTTGTCTGTGATGTCTAAGTGGCAGGTTCTTTTGTGCTCTT
TTGTATTTTTATATCCTTTGTTATTGTTTTTGTTAAGGGTAGGTTGCTGGAGCAGCAGCT
GCTGCTGGACTTTCTCTAGCAGAAGTTGCAGCAGAAGCAAAACATGCATCTGAAATGGTT
GGAACCATGGGTGTTGCACTATCTGTCTGTGCGCTTCCAGGACAGGTTACATCAGATCGT
TTGGGTCCAGAGAAAATGGAACTTGGGCTTGGAGTTGTATGTTGTTTCTCTTCTTATTTT
CTTTTATGACATAGGAAAGTCAGAGCATTACTCTTGCTAATATTTTGACCTTTTTTTGCT
CGCTGTTTTTCGACTTTTCTCTCAGCATGGGGAGCCTGGTGCTACAGAGAAAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 05-K109028-0022-278-C01C-8409
GenBank Accession LN488390 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K109028-0022-278-C01C-8409
GCCATTTTTCTCTGTAGCCCAGGCTCCCCATGCTGAGAGAAAAGTCGAAAACCATTTAGC
AAAAAAAGGTCAAAATATTAGCAAGAGTAATGCTCTGACTTTCCTATGTCATAAAAGAAA
ATAAGAAGAGAAACAACATACAACTCCAAGCCCAAGTTCCATTTTCTCTGGACCCAAACG
ATCTGATGTAACCTGTCCTGGAAGCGCACAGACAGATAGTGCAACACCCATGGTTCCAAC
CATTTCAGATGCATGTTTTGCTTCTGCTGCAACTTCTGCTAGAGAAAGTCCAGCAGCAGC
TGCTGCTCCAGCAACCTACCCTTAACAAAAACAATAACAAAGGATATAAAAATACAAAAG
AGCACAAAAGAACCTGCCACTTAGACATCACAGACAAATATAAGAAAATGCAATAATAAA
AAAAGATGAGGATAAGTGACTGCAAACACTACTAAGAAATGCTATCTTATAGTGCAGATA
GAGGAAAAACAACATAGAAGAAAACTGAGAACCTTATGGACAAGAACTGTTCCCGCTAAA
CCTCTGCGCCCAGCTACGCCACGCGGCGGGGGTAGAGCACAATCATCTCCAACA

Last Updated on 06.12.2018 16:11