Sequences from confirmation amplicon


Confirmation sequences for line 278H07 and hit At5g38396 are shown.
Line ID 278H07
Locus At5g38396 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15374630
Primers used for PCR 18rn - 8474, show primer details
   
Sequence Name 48-K116204-0022-278-H07C-18rn
GenBank Accession LN506609 [GenBank]
Sequencing primer gene specific primer, 18rn: 5'-TAAATAACGCGTGAAATCTTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116204-0022-278-H07C-18rn
AAATGGTAACGACTGCTTTCGAATGCTTGTTTGGGATTGCGTTGTTTTCTATTATTCTGT
TCTACCTGCTAGATTTCACGGGACCAATAATAGGCTTATTTCTCATGGTAGTAAAAACCT
ATGCTTTAGTTTTGTTCTTCAGAGGCTTCATTGTGCAGTGCAGTCGTATTCTTATTCATG
GTAACCACTAACCATAAGCTGGACATTGCTACTCGATGATTTGTTGTAGAGTTTTATCTT
CTCCGCGATAACTTCAGACACTTCGCGCTGTGCATGATCATTCCCTTCAATATAGATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTAT
   
Sequence Name 12-K116203-0022-278-H07C-8409
GenBank Accession LN491736 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116203-0022-278-H07C-8409
GCGCGAAGTGTCTGAAGTTATCGCGGAGAAGATAAAACTCTACAACAAATCATCGAGTAG
CAATGTCCAGCTTATGGTTAGTGGTTACCATGAATAAGAATACGACTGCACTGCACAATG
AAGCCTCTGAAGAACAAAACTAAAGCATAGGTTTTTACTACCATGAGAAATAAGCCTATT
ATTGGTCCCGTGAAATCTAGCAGGTAGAACAGAATAATAGAAAACAACGCAATCCCAAAC
AATGCATTCGAAAGCAGTCTGTTACCATTTTTTCTCCTTCAACTCAATTATGTTTATAAG
CCAACAAGATTTCACGCGATTATTTA

Last Updated on 06.12.2018 16:11