Sequences from confirmation amplicon


Confirmation sequences for line 279E01 and hit At1g11180 are shown.
Line ID 279E01
Locus At1g11180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3747769
Primers used for PCR 77zl - 8474, show primer details
   
Sequence Name 12-K130850-0022-279-E01C-77zl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 77zl: 5'-GTATGTGGTTCAAGACTCGCGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K130850-0022-279-E01C-77zl
GTACACACAACGATAATAGTTTGCAAGTTTTTACTAAATTGAATTCAATTTTTTATACAT
GAAGCTTTACTTTTACTACTTTTAATAACAGCTGACGAAAATAAAAATAAAAGTTCAAGA
AAAAAAATCGATCAGTGAAGCTAAATAACAACTGAAAAATAGTGAAAAACATTACACATC
ACAAGCAATGGCTTAAGAAGAGATGACTAGACTCATTGATTTACATTTGCCTCATATGGC
AGCTCTCATGGCTCCTCTTGCAGCATCCCGTCTCATATCATCCGCTTTTCCGCTTCCTCT
GAAGTACATGTATACTTGCTGCACACAATCAAATGTTAAAAAGAGTTTTGCTTCAGAACC
TATATCGATCCAGAGATTGTATCTGATTAGGAAAGAGACCTGTATAACCCAGATGCTAAC
CACTGATTCAAGACAGAATAACCCAAACCCAATGAAGTAGAAGATCTGTAAAAAGGAAAA
AAACAAGGTATTATCGATGAGTGATAGTATGATCAGGAGCCTCTCTACGTTTATCAGGCA
AGATTTATGGTTGCAGCCGAAAAATCAAGGGATGGATAAGGGCTTACCCCGACCAATGCC
TGAGCGCTCAACACATCTATTGCAGGTAATATGCCCCTGTATATTTCAAGATCATCAGAC
ACTTGAACATTAACTGTGTTACACCCTAGACGATGTCTTAAAGCTTGTGAGGAAACTTAC
GCAAGTGATTTTCCTTTGAAGACAATAGGAGGAGCAACTGCAGCAAAGAGTATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
   
Sequence Name 03-K130849-0022-279-E01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K130849-0022-279-E01C-8409
TTGAATATACTCTTTGCTGCAGTTGCTCCTCCTATTGTCTTCAAAGGAAAATCACTTGCG
TAAGTTTCCTCACAAGCTTTAAGACATCGTCTAGGGTGTAACACAGTTAATGTTCAAGTG
TCTGATGATCTTGAAATATACAGGGGCATATTACCTGCAATAGATGTGTTGAGCGCTCAG
GCATTGGTCGGGGTAAGCCCTTATCCATCCCTTGATTTTTCGGCTGCAACCATAAATCTT
GCCTGATAAACGTAGAGAGGCTCCTGATCATACTATCACTCATCGATAATACCTTGTTTT
TTTCCTTTTTACAGATCTTCTACTTCATTGGGTTTGGGTTATTCTGTCTTGAATCAGTGG
TTAGCATCTGGGTTATACAGGTCTCTTTCCTAATCAGATACAATCTCTGGATCGATATAG
GTTCTGAAGCAAAACTCTTTTTAACATTTGATTGTGTGCAGCAAGTATACATGTACTTCA
GAGGAAGCGGAAAAGCGGATGATATGAGACGGGATGCTGCAAGAGGAGCCATGAGAGCTG
CCATATGAGGCAAATGTAAATCAATGAGTCTAGTCATCTCTTCTTAAGCCATTGCTTGTG
ATGTGTAATGTTTTACACTATTTTTCAGTTGTTATTTAGCTTCACTGATCGATTTTTTTT
CTTGAACTTTTATTTTTATTTTCGTCAGCTGTTATTAAAAGTAGTAAAAGTAAAGCTCAT
GTATAAAAAATTGAATTCAATTTAGTTAAAAACTTGCAAACTATTTATCGTTTGTTGTGT
TACTTGTTATATCTCCAAAATATATATCGCGAGTCTG

Last Updated on 06.12.2018 16:11