Sequences from confirmation amplicon


Confirmation sequences for line 280C07 and hit At5g11790 are shown.
Line ID 280C07
Locus At5g11790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3801301
Primers used for PCR SP21 - 8474, show primer details
   
Sequence Name 32-K103556-0022-280-C07C-SP21
GenBank Accession LN500550 [GenBank]
Sequencing primer gene specific primer, SP21: 5'-TGCAGATATGTTCTGTTTTCAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103556-0022-280-C07C-SP21
TCCACAACTTTTGCATATATCACATCAGTCCTCTTGGCCATGAGGTTGGTAACTGGACTC
TGATCGTTTACAAACTTTTGTATGTTTCTGGTATATTACTGTTAAACGCTATGACATTCC
TATGATAATGCAATTCATATCCCTTATTAAATTGCTTTCTTCAAGAGTCATTGCAGTTGG
GAGCTCCAATGATCAGTGTTGATGCTCCTTTGCTCTCAGCTGATGACTTAGCAGACCAGA
TAGTCGAGGTTCTTAATTATTTCGGGTAAGCGAATTCATCTATCACATAAGTTTTACTTG
TGATTGTTATTTCCTTGAGCTGCCTAGATTCTGGTTATATCGTTATCTTCGTTCGTTGCC
AAAGTCTTTTTCCGGAGACTAGGCAGAAGAAAGAAAACCAAAACTTTTTTGCCTATATAG
AAATAGAATTTTCTCCTTATTTATTTCTTGTCGTCTCTTGTCCAGACTTGGTGCAGTAAT
GTGTATGGGTGTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 94-K103557-0022-280-C07C-8409
GenBank Accession LN515305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K103557-0022-280-C07C-8409
CCCATACACATTACTGCACCAAGTCTGGACAAGAGACGACAAGAAATAAATAAGGAGAAA
ATTCTATTTCTATATAGGCAAAAAAGTTTTGGTTTTCTTTCTTCTGCCTAGTCTCCGGAA
AAAGACTTTGGCAACGAACGAAGATAACGATATAACCAGAATCTAGGCAGCTCAAGGAAA
TAACAATCACAAGTAAAACTTATGTGATAGATGAATTCGCTTACCCGAAATAATTAAGAA
CCTCGACTATCTGGTCTGCTAAGTCATCAGCTGAGAGCAAAGGAGCATCAACACTGATCA
TTGGAGCTCCCAACTGCAATGACTCTTGAAGAAAGCAATTTAATAAGGGATATGAATTGC
ATTATCATAGGAATGTCATAGCGTTTAACAGTAATATACCAGAAACATACAAAAGTTTGT
AAACGATCAGAGTCCAGTTACCAACCTCATGGCCAAGAGGACTGATGTGATATATGCAAA
AGTTGTGGAGTAACAAAGAACTAGCTTCTGGACAAAACAATAATCCTTGAAAACAGAAAT
TATCTGC

Last Updated on 06.12.2018 16:11