Sequences from confirmation amplicon


Confirmation sequences for line 280G05 and hit At2g18196 are shown.
Line ID 280G05
Locus At2g18196 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7921478
Primers used for PCR 31xy - 8474, show primer details
   
Sequence Name 77-K119830-0022-280-G05C-31xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 31xy: 5'-ACTGTTTGTTCTTTCGCTATCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K119830-0022-280-G05C-31xy
CCCTCACCGTTACCACACATGCCAATGGCTCGTCCACTTTCTTTACAGGTATTCTCTTTC
CTTTTTATGTATCTATATAGTATAATCGACCATAATTTTCATGTATCTATGTAGTTTTAG
TCTCAAATTGACTCTATGATCATGATATTAGTTTTTCATTAAAATAGAATAAATTTTGGT
AACAATTATCTTTCAAACCGTTATTATTCTGATACATGCCTAATTGAACGAAGAGTGCAA
ATGATTTTTGTTTTTAGAGAAAGGTATGACTTTGTTAGGTTTGTGATGATGGTAGCTAGA
GCGAATTAAGTTGCTTTTTTTTTTTAGCTAAACATAAAGATCAAAGTTCAAACTATAGTT
AGCTGATCTCCTCTAATTTTGTAAATAAGTTGGCAAAACAAATTCTCGATCGCAAAAAGA
ATATTAAAGTACTCATTGGGCCCATTTTTTTTTTCCCCTTTGGTAAGTAAAAAAAGGCCT
TTCCAATTTTTATGGATCCTTTAAGGGAAATCGAAAAAAACTAAAACGAAAGGTCAAAAT
TTTGTCCCACAAGGGAAGGTTACAATTTGGAATTAAAAAACCCTTGGGCGAATTCACCAA
AAAGTGGGCCTTTAAAAAAATTATCCTTCAAATAAATTCCCTTTTTCTTTAGGGACATAT
TTCACATATAAATCCAAATTGTTTTTGAACCATTCATATAACCAGGGGCTATTTAAATAC
AAATTGTTTTTAAATTCATATGGAATATTTCCATTTGAAAAGGGTTTCATGTCCGGAA
   
Sequence Name 53-K119829-0022-280-G05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K119829-0022-280-G05C-8409
TTACATTGATATATTCATATGACTTCAAAACAATTAGTATTAATATAGCCATTGGTAATA
TGACTGCTTCAAAAACAATTAGTATTAATATGTGATATATGTCCATAAAGAAAAAGGTAA
TTAATTAGAAGAATAATTATTTAAATGACCAACTCTCTGCTGAATCAGCCAAAGGATATT
ATAATCACATATAGTAACATCCACTAGTGAGACAAACTTATGACATTCCGTTTCAGTTTT
GTCCGATTCCCCTAAACGAATCAATAAAAATTGAAAAGACATTTTTTAACTAACAAAAGA
GAAAAAAAAAAATGAGCACAATGAGTACTTTAATATTCTTTTTGCGATCGAAAATCTGTT
TTGCCAACTTATTTACAAAATTAGAGGAGATCAGCTAACTATAGTTTGAACTTTGATCTT
TATGTTTAGCTAAAAAAAAAAAGCAACTTATTTCCCTTTAGCTCCCATCACCACAAACCT
AAAAAAGTCTTACTTTTCTCTAAAAACAAAAATCATTTGCCCTCTCCTTTCAATTAGGCG
TGTATCAAAAAAAAAACGGTTTAAAAAAAAATTGTTCCCAAAATTTTTTCTTTTTTAAGG
AAAAACTAAATTCAGGATCATAGAGCCATTTTGAAACTAAACCTCCTTAAATCCTTGAAA
TTTTGGGCCGATTATCCTATTTGGATCCATAAAAGGGAAAAAAAATCCCTGTAAAGAAAT
TGAACAACCCTTTGCCATGGTGGGGTAAGGGTTAAGGGGCCTCAAACTTTT

Last Updated on 06.12.2018 16:11