Sequences from confirmation amplicon


Confirmation sequences for line 281A06 and hit At2g36680 are shown.
Line ID 281A06
Locus At2g36680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15370515
Primers used for PCR CL80 - 8474, show primer details
   
Sequence Name 51-K032927-022-281-A06C-8409
GenBank Accession LN507407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K032927-022-281-A06C-8409
ATTTACAATTGATGACATATTACTGTTTTGTTAGTTGCTGCTAACATAGTTAAGGTGAAC
AAAAATTTTAGTTGTAATCATTAAAAGAACCTGATCTATGCTCATGAAGTTGTGCTGGGT
CTTAAGATTTTGCAATGTTAGTATATTGGGTATTGGAACAGTTTCAGGTAAGTATTTTTA
AGATTGTTGTGTTAAAAATCCAGGGGATCAAAAGAACAGCAACAAGGACAATCTCGTCCT
TCTCCAGAAGCTTCTGCAACACCGTGGTATTCGCCGTCTCTTGTCACCTCTCCTAGCTCA
TCCCGGCCTCAAACCTCAGGGCAGATTCCATCACATGTTTCACCAGGTGAAGCCGCCGGC
ATTATTGCCATTTTAAAAGACAAAAGGTTAGTCTTCAATTTCCTTATACCTTCTTGTTTG
CCTCTTTTGTACATGATGTATAACAGTTTTCTTGTGTCACACAGTCACACATTTCTTGTC
ATCTTTAATATGAATTTATGGTCTTTTAGGCTTGGAATAGTTTAGATTAACGCTAGACCA
AACACTAGATTTACTGGTTCTGTGGTTTTGATAGTTTGGTTGCTGATAGTCTCTTGTGAT
TTTG
   
Sequence Name 51-K032927-022-281-A06C-CL80
GenBank Accession LN507408 [GenBank]
Sequencing primer gene specific primer, CL80: 5'-AGTAACACAACGCAAAATCACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K032927-022-281-A06C-CL80
CAAACCCAGAACCAGTAAATCTAGTGTTTGGTCTAGCGTTAATCTAAACTATTCCAAGCC
TAAAAGACCATAAATTCATATTAAAGATGACAAGAAATGTGTGACTGTGTGACACAAGAA
AACTGTTATACATCATGTACAAAAGAGGCAAACAAGAAGGTATAAGGAAATTGAAGACTA
ACCTTTTGTCTTTTAAAATGGCAATAATGCCGGCGGCTTCACCTGGTGAAACATGTGATG
GAATCTGCCCTGAGGTTTGAGGCCGGGATGAGCTAGGAGAGGTGACAAGAGACGGCGAAT
ACCACGGTGTTGCAGAAGCTTCTGGAGAAGGACGAGATTGTCCTTGTTGCTGTTCTTTTG
ATCCCCTGGATTTTTAACACAACAATCTTAAAAATACTTACCTGAAACTGTTCCAATACC
CAATATACTAACATTGCAAAATCTTAAGACCCAGCACAACTTCATGAGCATAGATCAGGT
TCTTTTAATGATTACAACTAAAATTTTTGTTCACCTTAACTATGTTAGCAGCAACTAACA
AAACAGTAATATGTCATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11