Sequences from confirmation amplicon


Confirmation sequences for line 281C04 and hit At3g15450 are shown.
Line ID 281C04
Locus At3g15450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5213820
Primers used for PCR PP03 - 8474, show primer details
   
Sequence Name 14-K102660-0022-281-C04C-8409
GenBank Accession LN492459 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102660-0022-281-C04C-8409
CCATTTACATTGAAACATACATCCTGCAATAACAAAAACACAAAACACTCATCAAGATCT
AAACTCAAAAGTATGTTTCTTTCTTTATAGGGCTAATGAAAAACTTAAGTTTTGGTTTAC
CATTAGGGAAAGGAGCAAACGATTTAGCACAGCCTTGCTTTATGATCTGAATATCATCAG
ACATTACAACAGATCCGTCTCCAGAAATTCCCCAGTAAAGACTCTCTCCTCCATCAGAAC
TCAGAGCTGAGAAAACAGAGGAAGTTTGAGTATCGTAGACAACGAAAGCGAAGCTTCCCT
CAAGACCTCTAAGAACTTGATCTGCTGGGTAAGGACCACGATCACGAAGTGTTCGATAAG
CTTCGATCACAAACATTGCCTCGTTTGAGTTCTTCCCAGATAAACCGTATTGTCGATTCA
ATGTACAGAGATTATTCAATCTCCCTAGAAACATACAGTAGATCCCATCTAGTCCACAGA
ACAACCTGAGCAAAACAACAAAACCATTATGAATCTATGAATTGGATTTAGAGATTTGAC
TTGTATATATGTGTTTTATGTATATTAGTATATACCTTTGACGAAGAGAGGTTTCTTGGC
GAGCGTAAGCTAAGACGGCGGAGTCGCCGAAGTTCATAGAGAAAGCATTGTTTTGGTGA
   
Sequence Name 33-K102661-0022-281-C04C-PP03
GenBank Accession LN500950 [GenBank]
Sequencing primer gene specific primer, PP03: 5'-GACTTCCTCTCTCATCACCAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K102661-0022-281-C04C-PP03
CGTCTTAGCTACGCTCGCCAGAAACCTCTCTTCGTCAAAGGTATATACTAATATACATAA
AACACATATATACAAGTCAAATCTCTAAATCCAATTCATAGATTCATAATGGTTTTGTTG
TTTTGCTCAGGTTGTTCTGTGGACTAGATGGGATCTACTGTATGTTTCTAGGGAGATTGA
ATAATCTCTGTACATTGAATCGACAATACGGTTTATCTGGGAAGAACTCAAACGAGGCAA
TGTTTGTGATCGAAGCTTATCGAACACTTCGTGATCGTGGTCCTTACCCAGCAGATCAAG
TTCTTAGAGGTCTTGAGGGAAGCTTCGCTTTCGTTGTCTACGATACTCAAACTTCCTCTG
TTTTCTCAGCTCTGAGTTCTGATGGAGGAGAGAGTCTTTACTGGGGAATTTCTGGAGACG
GATCTGTTGTAATGTCTGATGATATTCAGATCATAAAGCAAGGCTGTGCTAAATCGTTTG
CTCCTTTCCCTAATGGTAAACCAAAACTTAAGTTTTTCATTAGCCCTATAAAGAAAGAAA
CATACTTTTGAGTTTAGATCTTGATGTGTGTTTTGTGTTTTTGTTATTGCAGGATGTATG
TTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACG
TGTCTAC

Last Updated on 06.12.2018 16:11