Sequences from confirmation amplicon


Confirmation sequences for line 281E12 and hit At3g28100 are shown.
Line ID 281E12
Locus At3g28100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10458803
Primers used for PCR 33xy - 8474, show primer details
   
Sequence Name 30-K119596-0022-281-E12C-33xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 33xy: 5'-GATTCTCTATTTGGCCTAGGTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K119596-0022-281-E12C-33xy
ATTTTCCTCGGTTAGTCTGTTGTATTTAAAAATTCTGCCTCCGATAACAAAATTAGTCTC
TTTTGCATGGAACATATCCAACACTTTGTATTATTTCTATTATGTATAAGAACAAAAATT
TGTACCTGGAGAATGAAAGAAACAGAAACAAAGATGTCTCTTATGGTAAGAAGAGCTCCG
CCGATGAGCCAATCTGAATTTGAAGATGATAACGGCGGAGAAAGCTGACGGAAGTTTATA
TACGGCGGAGAAGATGCAACAAAGACACGTGGACCATGGTAGAGAACCACAACAAGTGCG
CCTATTAACGACAATATCGTCCCCATCACTTTGGCTACATAATTACCAATGAGCAATTGA
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGG
TTATT
   
Sequence Name 06-K119595-0022-281-E12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K119595-0022-281-E12C-8409
ATTTTCATTGCTCATTGGTATTATGTAGCCAAGTGATGGGGACGATATTGTCGTTAATAG
GCGCACTTGTTGTGGTTCTCTACCATGGTCCACGTGTCTTTGTTGCATCTTCTCCGCCGT
ATATAAACTTCCGTCAGCTTTCTCCGCCGTTATCATCTTCAAATTCAGATTGGCTCATCG
GCGGAGCTCTTCTTACCATAAGAGACATCTTTGTTTCTGTTTCTTTCATTCTCCAGGTAC
AAATTTTTGTTCTTATACATAATAGAAATAATACAAAGTGTTGGATATGTTCCATGCAAA
AGAGACTAATTTTGTTATCGGAGGCAGAATTTTTAAAATACAACAGACTTAACCGAGGAA
AATTAGTTCAACCGTAAACCCACTGGATCCTCGAACCTAGGCCAAATAGAGAAT

Last Updated on 06.12.2018 16:11