Sequences from confirmation amplicon


Confirmation sequences for line 282A07 and hit At5g01530 are shown.
Line ID 282A07
Locus At5g01530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:209519
Primers used for PCR IH18 - 8474, show primer details
   
Sequence Name 75-K033215-022-282-A07C-IH18
GenBank Accession LN513175 [GenBank]
Sequencing primer gene specific primer, IH18: 5'-CATCACCAGATAACGCAGAGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K033215-022-282-A07C-IH18
ATGGATTTGTGAATCGATCCAACGGTCATAATCGTCTTTCCAGATAGGCCAGCTCCGAAA
CTCACCTCCACGCATCTATAATCGTGTATCAAGAAACGGATCTTTCTTCTCTATCTTCTG
AATATCACAAAACCCAATTAGCCGGAGATGGCCGCAACATCCGCCGCTGCTGCAGCTGCT
TCTTCCATCATGGGTACTCGGGTGGCTCCCGGTATCCATCCCGGTTCAGGTCGGTTCACA
GCCGTGTTCGGTTTCGGAAAGAAGAAGGCAGCTCCCAAAAAGAGTGCCAAAAAGACGGTG
ACTACGGACCGGCCTCTTTGGTACCCAGGCGCCATTTCTCCTGACTGGCTTGATGGTTCC
TTGGTTGGAGATTACGGTTTTGATCCCTTCGGTTTAGGCAAACCGGCCGAGTATCTTCAA
TTCGATATCGATTCACTAGACCAGAATCTGGCTAAGAACTTGGCCGGAGACGTGATCGGA
ACCCGTACGGAAGCTGCCGACGCCAAATCGACGCCGTTTCAGCCGTACAGTGAGGTGTTC
GGAATCCAGAGATTCAGGGAATGCGAACTCATCCACGGACGGTCAAACCGAAGGCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 67-K033215-022-282-A07C-8409
GenBank Accession LN511742 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K033215-022-282-A07C-8409
CGCCTTCGTTTGACCGTCCGTGGATGAGTTCGCATTCCCTGAATCTCTGGATTCCGAACA
CCTCACTGTACGGCTGAAACGGCGTCGATTTGGCGTCGGCAGCTTCCGTACGGGTTCCGA
TCACGTCTCCGGCCAAGTTCTTAGCCAGATTCTGGTCTAGTGAATCGATATCGAATTGAA
GATACTCGGCCGGTTTGCCTAAACCGAAGGGATCAAAACCGTAATCTCCAACCAAGGAAC
CATCAAGCCAGTCAGGAGAAATGGCGCCTGGGTACCAAAGAGGCCGGTCCGTAGTCACCG
TCTTTTTGGCACTCTTTTTGGGAGCTGCCTTCTTCTTTCCGAAACCGAACACGGCTGTGA
ACCGACCTGAACCGGGATGGATACCGGGAGCCACCCGAGTACCCATGATGGAAGAAGCAG
CTGCAGCAGCGGCGGATGTTGCGGCCATCTCCGGCTAATTGGGTTTTGTGATATTCAGAA
GATAGAGAAGAAAGATCCGTTTCTTGATACACGATTATAGATGCGTGGAGGTGAGTTTCG
GAGCTGGCCTATCTTGGAAAGACGATTATGACCGTTGGATCGATTCACAAATCCATCTGA
CGGTGAGTAGGATTACTAATCTTATCTATTAAACTCTGCGTTATCTGGTGATG

Last Updated on 06.12.2018 16:11