Sequences from confirmation amplicon


Confirmation sequences for line 282D03 and hit At3g54980 are shown.
Line ID 282D03
Locus At3g54980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20372313
Primers used for PCR 21tm - 8474, show primer details
   
Sequence Name 11-K116680-0022-282-D03C-21tm
GenBank Accession LN491287 [GenBank]
Sequencing primer gene specific primer, 21tm: 5'-AGGGTCTAAAGCCGAATAACTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116680-0022-282-D03C-21tm
AACAAAACGCTCTGGAAGTCGTCAATCATATGACTTCTTCCCAATATTGAAGTCAACGGG
GTTGTATACCAAACAATTATCAATGGTTTATGCAAAGTTGGTCAGACATCCAAAGCAAGA
GAATTGTTGGCAAATATGATCGAAGAAAAACGGTTATGTGTTAGTTGCATGAGTTACAAT
AGCATTATAGATGGATTTTTCAAAGAGGGTGAAATGGATTCTGCGGTTGCAGCATACGAA
GAGATGTGTGGCAATGGTATTTCTCCAAACGTCATAACTTATACCAGCTTGATGAATGGG
TTATGCAAAAATAACAGAATGGATCAAGCATTGGAAATGAGAGACGAAATGGCGAAAAAG
AAAATGGAAAGCGCATGTAATGAGAAAAAGAAAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGAT
   
Sequence Name 03-K116679-0022-282-D03C-8409
GenBank Accession LN487647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116679-0022-282-D03C-8409
CCTTTTTCTTTTTCTCTTACATGCGCTTTCCATTTTCTTTTTCGCCATTTCGTCTCTCAT
TTCCAATGCTTGATCCATTCTGTTATTTTTGCATAACCCATTCATCAAGCTGGTATAAGT
TATGACGTTTGGAGAAATACCATTGCCACACATCTCTTCGTATGCTGCAACCGCAGAATC
CATTTCACCCTCTTTGAAAAATCCATCTATAATGCTATTGTAACTCATGCAACTAACACA
TAACCGTTTTTCTTCGATCATATTTGCCAACAATTCTCTTGCTTTGGATGTCTGACCAAC
TTTGCATAAACCATTGATAATTGTTTGGTATACAACCCCGTTGACTTCAATATTGGAAGA
AGTCATATGATTGACGACTTCCAAAGCGTTTTGTTCATCATGATTCCTGAAACATCCATC
AATCAGAATGGAATACGTGTAGTTATTCGCTTTTTAGACCC
Primers used for PCR 22tm - 8474, show primer details
   
Sequence Name 14-K116680-0022-282-D03C-22tm
GenBank Accession LN492675 [GenBank]
Sequencing primer gene specific primer, 22tm: 5'-TCATCTCCTCAAACATCTTAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116680-0022-282-D03C-22tm
GTTACATTACTGTGTAAATGATTTCATCAGGGACAAGACCCACCGCCCCGCAAATCTGTG
TACAACTCAGAGGCCAGGATCAAATTTCCATCTTTCAACAATCCATCAATCAGTGTTGTA
TATGTACCCAGATCACATCGTAGACCGTCTTTCAACATTTTCTTGTACAAATCAAGGGCT
GCTACCATGTTTCCCAGATTACGAAATCCAGAAATCAAGCTGTTGTATATAGGTTGGCTT
GGATTCAATCCTTCTTCAAGGAGTTCAGAGAACAGAGCAGATGCGCTTTCCATGTTACTC
CTTTTGCAGAACCCATCGATAAGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 06-K116679-0022-282-D03C-oriL
GenBank Accession LN488979 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116679-0022-282-D03C-oriL
CTGCTCTGTTCTCTGCACTCCTTGAAGAAGGATTGAATCCACCCTAACCTATATACAACA
GCTTGATTTCTGGATTTCGTAATCTGGGAAACATGGTAGCAGCCCTTGATTTGTACAAGA
AAATGTTGAAAGACGGTCTACGATGTGATCTGGGTACATATACAACACTGATTGATGGAT
TGTTGAAAGATGGAAATTTGATCCTGGCCTCTGAGTTGTACACAGAAATGCAGGCGGTGG
GTCTTGTCCCTGATGAAATCATTTACACAGTAATTGTAAACGGTCTTAGCAAGAAGGGGC
AGTTTGTAAAAGTGGTTAAGATGTTTGAGGAGATGAACGAAAGA

Last Updated on 06.12.2018 16:11