Sequences from confirmation amplicon


Confirmation sequences for line 283E12 and hit At4g36950 are shown.
Line ID 283E12
Locus At4g36950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17422822
Primers used for PCR 14qz - 8474, show primer details
   
Sequence Name 38-K115806-0022-283-E12C-14qz
GenBank Accession LN503103 [GenBank]
Sequencing primer gene specific primer, 14qz: 5'-GAGAATGTTTTGGTTTTTGGTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115806-0022-283-E12C-14qz
CGAACGGAGGAGTGGTGAGGTTTGTGTTGAGATTAGAGGAACACCGTTGTATATGGCACC
GGAGTCTGTGAATCACGGCGAGTTTGAATCTCCGGCGGATATTTGGGCTTTGGGATGCTC
TGTTGTTGAGATGTCTAGTGGTAAAACGGCATGGTGTTTGGAGGATGGTGTTATGAATAA
TGTTATGTCTTTATTGGTTCGTATTGGTTCCGGCGATGAAGTTCCGAGGATTCCGGTGGA
ATTGTCGGAGGAAGGTAAAGATTTCGTGAGCAAGTGTTTTGTTAAAAACGCCGCGGAGAG
ATGGACGGCTGAGATGCTTTTGGATCATCCATTCTTAGCCGTTGATGATGAGAGTGGCGA
AGAAGACGAAGCTTGTTCGGTTTCACCGAGGAATCCATTTGATTTTCCCGGTTGGAATTC
GGTTCAATCTCCGGTTAATGATTCGGTTATGTTTGGTTCATTGGTAGGTTCGCCGGAGGA
AAGGATTAGTGGTTTGGTGAGTGAGAAGGTACCTGATTGGTCGGTGTCGTGTGATTGGGT
CAACGTCAGGTGAATAGAGTAGTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 17-K115805-0022-283-E12C-8409
GenBank Accession LN493996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115805-0022-283-E12C-8409
TTGAAACTACTCTATTCACCTGACGTTGACCCAATCACACGACACCGACCAATCAGGTAC
CTTCTCACTCACCAAACCACTAATCCTTTCCTCCGGCGAACCTACCAATGAACCAAACAT
AACCGAATCATTAACCGGAGATTGAACCGAATTCCAACCGGGAAAATCAAATGGATTCCT
CGGTGAAACCGAACAAGCTTCGTCTTCTTCGCCACTCTCATCATCAACGGCTAAGAATGG
ATGATCCAAAAGCATCTCAGCCGTCCATCTCTCCGCGGCGTTTTTAACAAAACACTTGCT
CACGAAATCTTTACCTTCCTCCGACAATTCCACCGGAATCCTCGGAACTTCATCGCCGGA
ACCAATACGAACCAATAAAGACATAACATTATTCATAACACCATCCTCCAAACACCATGC
CGTTTTACCACTAGACATCTCAACAACAGAGCATCCCAAAGCCCAAATATCCGCCGGAGA
TTCAAACTCGCCGTGATTCACAGACTCCGGTGCCATATACAACGGTGTTCCTCTAATCTC
AACACAAACCTCACCACTCCTCCGTTTCGCTAACCCGAAATCAGATATCTTCACATCTCC
ATCACCAAAAACCAAAACATTCT

Last Updated on 06.12.2018 16:11