Sequences from confirmation amplicon


Confirmation sequences for line 283G06 and hit At5g56420 are shown.
Line ID 283G06
Locus At5g56420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22851997
Primers used for PCR 22uw - 8474, show primer details
   
Sequence Name 61-K117914-0022-283-G06C-22uw
GenBank Accession LN510614 [GenBank]
Sequencing primer gene specific primer, 22uw: 5'-GACAGAGGTGATAGATCCCAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K117914-0022-283-G06C-22uw
ACATCGACATTTGCCTCCCCACCTCAGGCATGTTCTCAATGTAACAAAATCCACCCCAAT
AGTCTTCAATGTCTACACGCTTCAGTTTAGGAGTATCTATCAAGAACCCATCATCATCTC
CTTCAAAAGCTTGAGACGATTTATGCAAAGATAACGTTTTTAAACTAGGCGCAGTCACAG
TAACGACTTTAACATTGTCACCAGGGCATCTTTGCACAACCAAATCTTCAAGACTAGAAC
AGCTAGATATAATCCTAGGAAGTGACTGGTCATCTAAATACTTCACATCTAAAAGGTGCA
AAGTCTTGAGCGACTGGAAGCAAACATAACAAGAAGCATCTTCAAGAGTTATGTTTTCGA
GTTTCAGAACCGCAAGCGTTGAACAAGTAAACAAACACTTAGGCAATCTAATAGGCTCTT
CACCAGAACAATAACTAATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACGTGGATCATCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTAAA
   
Sequence Name 21-K117913-0022-283-G06C-8409
GenBank Accession LN495845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117913-0022-283-G06C-8409
ATTTCATTGAATATATCCTGAATTAGTTATTGTTCTGGTGAAGAGCCTATTAGATTGCCT
AAGTGTTTGTTTACTTGTTCAACGCTTGCGGTTCTGAAACTCGAAAACATAACTCTTGAA
GATGCTTCTTGTTATGTTTGCTTCCAGTCGCTCAAGACTTTGCACCTTTTAGATGTGAAG
TATTTAGATGACCAGTCACTTCCTAGGATTATATCTAGCTGTTCTAGTCTTGAAGATTTG
GTTGTGCAAAGATGCCCTGGTGACAATGTTAAAGTCGTTACTGTGACTGCGCCTAGTTTA
AAAACGTTATCTTTGCATAAATCGTCTCAAGCTTTTGAAGGAGATGATGATGGGTTCTTG
ATAGATACTCCTAAACTGAAGCGTGTAGACATTGAAGACTATTGGGGTGGATTTTGTTAC
ATTGAGAACATGCCTGAGGTGGTGGAGGCAAATGTCGATGTTATTTATAAAAATACGGAG
AAACTTCTGGGATCTATCACCTATAACAAAAAGAAAGATTCCTTTCCTTTTTTTTTTCAA
AAAGAAAATGTACCTCTCCACATCATAAAAA

Last Updated on 06.12.2018 16:11