Sequences from confirmation amplicon


Confirmation sequences for line 283G07 and hit At5g27260 are shown.
Line ID 283G07
Locus At5g27260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9604002
Primers used for PCR 43vh - 8474, show primer details
   
Sequence Name 40-K117953-0022-283-G07C-43vh
GenBank Accession LN503861 [GenBank]
Sequencing primer gene specific primer, 43vh: 5'-CATTCGACTGCAAAGAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117953-0022-283-G07C-43vh
TATGTTGGAAGTGTGATATTGATTAACTTTTGAATAGTTAACTTTTGTTTCCCGTCTCAC
TATTTCCTTGTTTTTTGCATTGACGTATGGTTATTTTTTATTAAAATATTGCAACTTTTT
CATGTTTTAACGTATATTTGTTTTTCCAATTTGTCATAGGTATCACTATGGGAGATTCAC
AACCCAAAAAAAGAAAAAAGGGGGATTATAATCCGTGGTCTCCAGAGATACCCGCAATGA
GTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 16-K117952-0022-283-G07C-8409
GenBank Accession LN493617 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117952-0022-283-G07C-8409
ATTGAATATATACTCATTGCGGGTATCTCTGGAGACCACGGATTATAATCCCCCTTTTTT
CTTTTTTTGGGTTGTGAATCTCCCATAGTGATACCTATGACAAATTGGAAAAACAAATAT
ACGTTAAAACATGAAAAAGTTGCAATATTTTAATAAAAAATAACCATACGTCAATGCAAA
AAACAAGGAAATAGTGAGACGGGAAACAAAAGTTAACTATTCAAAAGTTAATCAATATCA
CACTTCCAACATATGATTCTGTAAATAAGAACAAAAGAAAAAAGTAAGAACAAGTGAACC
TTTTTCTTTGCAGTCGAATG

Last Updated on 06.12.2018 16:11