Sequences from confirmation amplicon


Confirmation sequences for line 284A04 and hit At1g32100 are shown.
Line ID 284A04
Locus At1g32100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11546686
Primers used for PCR 15cv - 8474, show primer details
   
Sequence Name 71-K109031-0022-284-A04C-15cv
GenBank Accession LN512577 [GenBank]
Sequencing primer gene specific primer, 15cv: 5'-CGAGGTAGGGTTCAATCAGTAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K109031-0022-284-A04C-15cv
TCTTTTATTGGTTGTTAAAACCAAAGATAATATTTTTTCTTGCATGAAGAATATATTTTT
AAATTTGCATATATAGCAGAACATGAATGAATATAAAACATCAAACTTTTGATATGTGTA
TTTAGTATAGATAAGACTTGGTCAAATCAGAGGAACATTCTTAAGTAATCATCCATGCGT
TTGTACTTGACGTCAGGATAAAGACTAGAAGCTTCTTCGTCTTCTCCGACTTCGTGATCA
GTGAGACATCCTTCATAGAAAATGTGATAGAAATGTCCTATCCCTGCTTGGTGTGGAATC
TCCATTTCTACATACATGTAAAAGAAATCAAATAAAGTTGATGGGCTATATTTTTCAAAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATT
   
Sequence Name 66-K109030-0022-284-A04C-8409
GenBank Accession LN511630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K109030-0022-284-A04C-8409
GCCATTTACAATTGAATTATTTGAAAAATATAGCCCATCAACTTTATTTGATTTCTTTTA
CATGTATGTAGAAATGGAGATTCCACACCAAGCAGGGATAGGACATTTCTATCACATTTT
CTATGAAGGATGTCTCACTGATCACGAAGTCGGAGAAGACGAAGAAGCTTCTAGTCTTTA
TCCTGACGTCAAGTACAAACGCATGGATGATTACTTAAGAATGTTCCTCTGATTTGACCA
AGTCTTATCTATACTAAATACACATATCAAAAGTTTGATGTTTTATATTCATTCATGTTC
TGCTATATATGCAAATTTAAAAATATATTCTTCATGCAAGAAAAAATATTATCTTTGGTT
TTTAACAACCAATAAAAAGAACAACAACAACACAAGGAGTAAGGCGTTGGTAACTCTACT
GATTGAACCCTACCTC

Last Updated on 06.12.2018 16:11