Sequences from confirmation amplicon


Confirmation sequences for line 284H09 and hit At3g55770 are shown.
Line ID 284H09
Locus At3g55770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20703802
Primers used for PCR ML05 - 8474, show primer details
   
Sequence Name 26-K109642-0022-284-H09C-ML05
GenBank Accession LN497970 [GenBank]
Sequencing primer gene specific primer, ML05: 5'-TCGTTAATTGGGGAGAGATATACG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K109642-0022-284-H09C-ML05
CTTTTGCATCCTTACATCTCTCAATCTTTCTCTGGTTAATCTGTATTCGTGTATTTTTGC
AGTTTCTTTTGAGGATCAGATCGTGACAGGGTTTAGCTCAAGGAGAGGTATTAGATCCGT
ACTTTGTGATAAACTTTCTCAAGGTTTACGATCTCATTCTATCTAGATCGGCTTCGTATA
GATACAATCTTGTTTCAATTAGCAATGGATCGATTTTGTTATTCGAATATTTAGAAAAAG
GAAAGATTTTTCCAAAGGGGTTTCCTCTTATACCATTTTTTCTGGGTTTTTTGAAATGTA
GGGTGAGAAAAAGAAGAAGAAGGTGAGAAAAGTGAAGGCAAGGCTTTGAGGATGTATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTA
   
Sequence Name 10-K109633-0022-284-H09C-8409
GenBank Accession LN490700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K109633-0022-284-H09C-8409
CGGACTGAGCCATTTACAATTGAATACATCCTCAAAGCCTTGCCTTCACTTTTCTCACCT
TCTTCTTCTTTTTCTCACCCTACATTTCAAAAAACCCAGAAAAAATGGTATAAGAGGAAA
CCCCTTTGGAAAAATCTTTCCTTTTTCTAAATATTCGAATAACAAAATCGATCCATTGCT
AATTGAAACAAGATTGTATCTATACGAAGCCGATCTAGATAGAATGAGATCGTAAACCTT
GAGAAAGTTTATCACAAAGTACGGATCTAATACCTCTCCTTGAGCTAAACCCTGTCACGA
TCTGATCCTCAAAAGAAACTGCAAAAATACACGAATACAGATTAACCAGAGAAAGATTTG
AGAGATGTAAGGATGCAAAAGTCAACTTCTTTGTAGAGAGAAGAGCTGTTACGTATATCT
CTCCCCAATTAACG
Primers used for PCR NJ36 - 8474, show primer details
   
Sequence Name 23-K109674-0022-284-H09C-NJ36
GenBank Accession LN496596 [GenBank]
Sequencing primer gene specific primer, NJ36: 5'-CTCATCATCGTCACATAGATCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109674-0022-284-H09C-NJ36
GTAACAGCTCTTCTCTCTACAAAGAAGTTGACTTTTGCATCCTTACATCTCTCAAATCTT
TCTCTGGTTAATCTGTATTCGTGTATTTTTGCAGTTTCTTTTGAGGATCAGATCGTGACA
GGGTTTAGCTCAAGGAGAGGTATTAGATCCGTACTTTGTGATAAACTTTCTCAAGGTTTA
CGATCTCATTCTATCTAGATCGGCTTCGTATAGATACAATCTTGTTTCAATTAGCAATGG
ATCGATTTTGTTATTCGAATATTTAGAAAAAGGAAAGATTTTTCCAAAGGGGTTTCCTCT
TATACCATTTTTTCTGGGTTTTTTGAAATGTAGGGTGAGAAAAAGAAGAAGAAGGTGAGA
AAAGTGAAGGCAAGGCTTTGAGGATGTATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 07-K109673-0022-284-H09C-8409
GenBank Accession LN489317 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109673-0022-284-H09C-8409
TCGGACATNAGCCATTTACATTGAATACATCCTCAAAGCCTTGCCTTCACTTTTCTCACC
TTCTTCTTCTTTTTCTCACCCTACATTTCAAAAAACCCAGAAAAAATGGTATAAGAGGAA
ACCCCTTTGGAAAAATCTTTCCTTTTTCTAAATATTCGAATAACAAAATCGATCCATTGC
TAATTGAAACAAGATTGTATCTATACGAAGCCGATCTAGATAGAATGAGATCGTAAACCT
TGAGAAAGTTTATCACAAAGTACGGATCTAATACCTCTCCTTGAGCTAAACCCTGTCACG
ATCTGATCCTCAAAAGAAACTGCAAAAATACACGAATACAGATTAACCAGAGAAAGATTT
GAGAGATGTAAGGATGCAAAAGTCAACTTCTTTGTAGAGAGAAGAGCTGTTACGTATATC
TCTCCCCAATTAACGAAAAAGGAGGATCTATGTGACGATGATGA

Last Updated on 06.12.2018 16:11