Sequences from confirmation amplicon


Confirmation sequences for line 285B07 and hit At1g10060 are shown.
Line ID 285B07
Locus At1g10060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3285008
Primers used for PCR 18cs - 8474, show primer details
   
Sequence Name 54-K108895-0022-285-B07C-18cs
GenBank Accession LN508505 [GenBank]
Sequencing primer gene specific primer, 18cs: 5'-GTCTTCTCCTCTGTACGCTTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K108895-0022-285-B07C-18cs
TTTTTTATCCACTGAGATCATATATTTGTGTGATTTTGGCTAAGATTTGAAGCTTTGCCT
GGCCATAGTTGAGAATTCCAGCAGCAGGGTTGAGCTCGATGTTGCCGTAACGGCTAAGGT
AACCCTGTTCGAAGTTTCCGTCTCTGCAACTTTTGGTGGCGAACATGAAATCTGTCCGTA
CAAGACTGAATCCAAGGTTGTCCCAATCTACATCAGCATATTCTTCATCCTCACTGCATC
ACAGGAAGAATATTTTGCCTATTGAAGCCTATTTTCTTATATGATAACTAGAATCCAAGA
CAAGGACTATTCATATTCCTAACAAAACACTAACTTTTAAACTTTTGTTTTTTTGGTTTA
AAACTTTTAATGGTTGGAAATGTAAAGCACTATTTTTTTAGATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 38-K108894-0022-285-B07C-8409
GenBank Accession LN503009 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K108894-0022-285-B07C-8409
TTACATTGAATCTAAAACCAGTGCTTTACATTTCCAACCATTAAACCCTTTAAAGCAAAA
AAACAAAAGTTTAAAAGTTAGTGTTTTGTCCTTAATATGAATAGTCCTTGTCTTGGATTC
TAGTTATCATATAAGAAAATAGGCTTCAATAGGCAAAATATTCTTCCTGTGATGCAGTGA
GGATGAAGAATATGCTGATGTAGATTGGGACAACCTTGGATTCAGTCTTGTACGGACAGA
TTTCATGTTCGCCACCAAAAGTTGCAGAGACGGAAACTTCGAACAGGGTTACCTTAGCCG
TTACGGCAACATCGAGCTCAACCCTGCTGCTGGAATTCTCAACTATGGCCAGGCAAAGCT
TCAAATCTTATCCAAAATCACACAAATATATGATCTCAGTTGTGATAAAAAACTACTTTT
CTGGTCGACTTTGCAGGGACTAATAGAGGGGATGAAAGCGTACAGAGGAGAAGA

Last Updated on 06.12.2018 16:11