Sequences from confirmation amplicon


Confirmation sequences for line 285B09 and hit At5g11940 are shown.
Line ID 285B09
Locus At5g11940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3849867
Primers used for PCR 27qa - 8474, show primer details
   
Sequence Name 55-K115305-0022-285-B09C-27qa
GenBank Accession LN508898 [GenBank]
Sequencing primer gene specific primer, 27qa: 5'-AGTATACCTTGGTGAAAAGGAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K115305-0022-285-B09C-27qa
AGATCCCTACTCGGAAGGTACCAACAAACCAAGATTAGACAAGTAAAACAGTATGTTGTT
GGAAGTAATATTTAACAGATTTTGTGGAAATATGGTTGCAGCAAAAAAGACGCTAGTGAG
TCCATAGTTCATAGTTATCGGAATGGTTTCTCCGGCTTTGCTGCCCATCTTACGGATTCC
CAAGCGGAACAAATCTCAGGTGTGTACATCTAATGACTTTTTAGTAGAAAGATATAGGAA
GCAAATTAGATCAAAGGTTAATTAATGATTTTTAATCTTCATAATTATGATTAGAGCATC
CAGATGTAGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCA
   
Sequence Name 20-K115304-0022-285-B09C-8409
GenBank Accession LN495342 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115304-0022-285-B09C-8409
ATTTACATTGAACACATCTGGATGCTCTAATCATAATTATGAAGATTAAAAATCATTAAT
TAACCTTTGATCTAATTTGCTTCCTATATCTTTCTACTAAAAAGTCATTAGATGTACACA
CCTGAGATTTGTTCCGCTTGGGAATCCGTAAGATGGGCAGCAAAGCCGGAGAAACCATTC
CGATAACTATGAACTATGGACTCACTAGCGTCTTTTTTGCTGCAACCATATTTCCACAAA
ATCTGTTAAATATTACTTCCAACAACATACTGTTTTACTTGTCTAATCTTGGTTTGTTGG
TACCTTCCGAGTAGGGATTCTAACATTCTGAGATGCGAGCTGGTTACAAGCTCAGGATCA
TTATGCTCCTTTTCACAGAGGTATAC

Last Updated on 06.12.2018 16:11